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- Clone
- A21110A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ARTN, ART, Enovin, Neublastin, EVN, NBN
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant human GFRα3/GFRA3-Fc chimera (Cat. No. 780406) (black circles) binds to immobilized recombinant human Artemin (Cat. No. 780206). Ultra-LEAF™ purified anti-human Artemin (clone A21110A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2b, κ isotype control (Cat. No. 401216) (gray triangles) does not have this effect. This antibody blocks the binding of 0.2 µg/mL human GFRα3/GFRA3-Fc chimera to 1 µg/mL immobilized recombinant human Artemin. ND50 range: 0.2-1.2 µg/mL
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 621553 | 100 µg | 328€ | ||||
| 621554 | 1 mg | 972€ | ||||
Artemin belongs to the GDNF family of ligands consisting of 4 members: GDNF, Neurturin, Artemin, and Persephin. GDNF family members orchestrate multiple aspects of the development and maintenance of the central and peripheral nervous systems. These GDNF family ligands signal through a multicomponent receptor system composed of a common signaling component RET (receptor tyrosine kinase) and one of the high affinity binding coreceptors GFRα (α1-α4). Artemin is a GDNF family member that functions as a ligand for the GFRα3 and RET complex. Artemin is a disulfide-linked homodimer that contains three intra-chain disulfide linkages and a cysteine knot motif. Artemin supports the survival of sensory and sympathetic peripheral neurons and also supports survival of dopaminergic midbrain neurons. Artemin expression in adult tissues is ubiquitous with high levels in peripheral tissues including the prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. In adult rat, systemic artemin treatment promoted the regeneration of sensory axons to the brainstem after brachial dorsal root crush. Recent findings suggest Artemin regulates multiple aspects of carcinoma progression and metastasis. Artemin is a hypoxia-responsive factor and is essential for hypoxia-induced cancer stem cell expansion in hepatocellular carcinoma and Artemin stimulates de novo tumor angiogenesis in mammary carcinoma mediated in part by VEGF-A.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human Artemin
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.2 µg/mL human GFRα3/GFRA3-Fc chimera to 1 µg/mL immobilized recombinant human Artemin. ND50 range: 0.2-1.2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3083410 (BioLegend Cat. No. 621553)
AB_3083410 (BioLegend Cat. No. 621554)
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis
- Function
- Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons
- Ligand/Receptor
- CGFRα3, GFRα1
- Cell Type
- Embryonic Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Adhesion Molecules, Growth Factors
- Antigen References
-
- Baloh RH, et al. 1998. Neuron. 21:1291-1302
- Masure S, et al. 1999. Eur J Biochem. 266:892-902
- Gardell LR, et al. 2003. Nature Med. 9:1383-1389
- Honma Y, et al. 2002. Neuron. 35:267-282
- Arindam B, et al. 2012. PLoS One. 7:e50098
- Wong LE, et al. 2015. Proc Natl Acad Sci USA. 112:6170-5
- Zhang M, et al. 2016. Oncotarget. 7:3267-82
- Gene ID
- 9048 View all products for this Gene ID
- UniProt
- View information about Artemin on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All Artemin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human Artemin | A21110A | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human Artemin
Recombinant human GFRα3/GFRA3-Fc chimera (Cat. No. 780406) (...
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