Purified anti-human HB-EGF Antibody

Pricing & Availability
3H4 (See other available formats)
Regulatory Status
Other Names
Heparin-binding EGF-like growth factor, HB-EGF-like growth factor
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
NUGC-3 cells (human gastric cancer cells) were stained with purified anti-human HB-EGF (clone 3H4) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
  • 3H4_PURE_HB-EGF_Antibody_082622
    NUGC-3 cells (human gastric cancer cells) were stained with purified anti-human HB-EGF (clone 3H4) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
Compare all formats
Cat # Size Price Quantity Check Availability Save
383702 100 µg 308€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of growth factors. Human HB-EGF was initially identified as a protein of 22 kD secreted by macrophage-like U937 cells. It belongs to the EGF family of proteins that includes EGF, TGF-α, HB-EGF, epigen, epiregulin, betacellulin, neuroregulin, and tomoregulin. All the EGF family members are synthesized as type I membrane protein precursors, which can undergo proteolytic cleavage at the plasma membrane to release a mature soluble ectodomain. It has been suggested that various metalloproteinases participate in the shedding of HB-EGF, such as MMP-3, MMP-7, ADAM9, ADAM10, ADAM12, and ADAM17. The ectodomain shedding is stimulated by phorbol esters, calcium ionophore, lysophosphatidic acid, and IL-1β. In addition, nardilysin, a metalloendopeptidase of the M16 family, binds HB-EGF and enhances its shedding through activation of TACE (ADAM17). The membrane-anchored HB-EGF acts in cell-cell and cell-extracellular matrix interactions. In addition, the membrane HB-EGF is the receptor for diphtheria toxin that can be internalized and induce apoptotic death. HB-EGF plays a crucial role in cardiac valvulogenesis. Newborn knockout mice have malformed semilunar and atrioventricular heart valves and poorly differentiated lungs. HB-EGF is overexpressed in several types of cancer such as pancreatic carcinoma, ovarian cancer, and gastric carcinoma. HB-EGF is also involved in cancer metastasis and invasion of ovarian cancer, head and neck cancer, and thyroid carcinoma cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human HB-EGF, extracellular domain (recombinant)
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
ICC, WB, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Hamaoka M, et al. 2010. J Biochem. 148:55.
AB_2927862 (BioLegend Cat. No. 383702)

Antigen Details


Monocytes, macrophages, T cells, neutrophils, eosinophils, myeloid leukemia blasts, myeloma cells, vascular smooth muscle cells, endothelial cells, and normal or neoplastic epithelial cells

HB-EGF is associated with proliferation and differentiation of stromal cells, apoptosis, and morphogenesis. It participates in inflammation, wound healing, atherosclerotic plaque progression, and cardiac valvulogenesis. TNF-α and IL-1β induces HB-EGF in endothelial cells.
Fibroblast, smooth muscle cells, monocytes/macrophages, endothelial cells, astrocytes, keratinocytes, normal and neoplastic epithelial cells
EGFR (ErbB1) and ErbB4; also binds to heparan-sulphate proteoglycans
Cell Type
Epithelial cells, Macrophages, T cells
Biology Area
Angiogenesis, Cancer Biomarkers
Molecular Family
Growth Factors
Antigen References
  1. Higashiyama S, et al. 1991. Science. 251:936.
  2. Hirata M, et al. 2001. Biochem. Biophys. Res. Commun. 283:915.
  3. Jackson LF, et al. 2003. EMBO J. 22:2704.
  4. Nishi E, et al. 2006. J. Biol. Chem. 281:31164.
  5. Yotsumoto F, et al. 2008. Biochem. Biophys. Res. Commun. 365:555.
  6. Ota I, et al. 2013. Oncol Rep. 30:1593.
Gene ID
1839 View all products for this Gene ID
View information about HB-EGF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08-26-2022

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account