Purified anti-β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39320)

Pricing & Availability
Clone
6E10 (See other available formats)
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9320-02
Signet Catalog# 9320-05
Signet Catalog# 9320-10
Covance Catalog# SIG-39320
Isotype
Mouse IgG1
Ave. Rating
5 reviews
Product Citations
publications
A_6E10_PURE_betaAmyloid_1-16_WB_050818
Western blot of purified anti-β-amyloid, 1-16 antibody (clone 6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide; Lane 4: 50 ng of the recombinant human APP751 protein; Lane 5: 20µg of the human brain lysate; Lane 6: 50 ng of the rodent Aβ1-42 peptide. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A_6E10_PURE_betaAmyloid_1-16_WB_050818
    Western blot of purified anti-β-amyloid, 1-16 antibody (clone 6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide; Lane 4: 50 ng of the recombinant human APP751 protein; Lane 5: 20µg of the human brain lysate; Lane 6: 50 ng of the rodent Aβ1-42 peptide. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • B_6E10_PURE_betaAmyloid_1-16_DirectELISA_050818
    Direct ELISA of purified anti-β-amyloid 1-16 (clone 6E10) antibody binding to the plate-immobilized human Aβ1-40 and the recombinant human APP751. ELISA was performed by coating the wells with 100 ng of peptide or recombinant protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with HRP labeled goat anti-mouse IgG secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • C_6E10_PURE_betaAmyloid_1-16_IHCP_050818
    IHC staining of purified anti-β-Amyloid, 1-16 antibody (clone 6E10) on the formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel) brain tissues. Following antigen retrieval using 70% formic acid for 20 minutes, the tissues were incubated with 1µg/mL of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
803004 25 µl 76€
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803001 200 µl 378€
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803002 500 µl 738€
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803003 1 ml 1.215€
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Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
Direct ELISA, IHC-P Validated
IHC-F, EM, ICC -  Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For Direct ELISA, the suggested use of this reagent is 0.002 - 0.02 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.2 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS).

This antibody clone has been reported for use in immunohistochemistry of free-floating sections2,13.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References
  1. Thakker DR, et al. 2009. Proc. Natl. Acad. Sci. USA. 106(11):4501-6. (IHC) PubMed
  2. Oddo S, et al. 2005. Proc. Natl. Acad. Sci. USA. 102(8):3046-51. (IHC-other) PubMed
  3. Herzig M, et al. 2004. Nat. Neuro. 7(9):954-959. (WB) PubMed
  4. Zheng Y, et al. 2012. PLoS One 6:39035. (IHC-F) PubMed
  5. Abramowksi D, et al. J Neurosci. 32:1273. (WB) PubMed
  6. Forny-Germano L, et al. 2014. J. Neurosci. 34:13629. (WB, IHC) PubMed
  7. Gowert NS, et al. 2014. PLoS One 2:e90523. (ICC, EM) PubMed
  8. Sandoval-Hernández A, et al. 2015. PLoS One. 10: 0145467. (IHC-F)
  9. Kumar R, et al. 2016. Brain. 139:174-92 (WB)
  10. Miyamoto T, et al. 2016. J. Biol. Chem. 291:1719-34. (WB)
  11. Saito S, et al. 2017. Acta Neuropathol. Commun. 5:26-9. (IHC-P) PubMed
  12. Omata Y, et al. 2016. Aging (Albany NY) 8(3):427. (IHC-P) PubMed
  13. Peng W, et al. 2016. Neurobiol. Dis. 93:215. (IHC-other) PubMed
  14. Mandler M, et al. 2015. PLoS One. e0115237. (WB, IHC, ELISA) PubMed
Product Citations
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  2. Kumar R, et al. 2016. Brain. 139: 174 - 192. PubMed
  3. Miyamoto T, et al. 2016. J Biol Chem. 291: 1719 - 1734. PubMed
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  7. Cifelli J, et al. 2016. J Biol Chem. 291: 11981 - 11992. PubMed
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  13. Hostetler E, et al. 2016. J Nucl Med. 57: 1599 - 1606. PubMed
  14. A Kimura, S Hata, T Suzuki 2016. J Biol Chem. 291: 24041 - 24053. PubMed
  15. Daria A, et al. 2017. EMBO J. 10.15252/embj.201694591. PubMed
  16. Flanagan M, et al. 2017. J Neuropathol Exp Neurol. 10.1093/jnen/nlw104. PubMed
  17. Yang S, et al. 2017. EMBO Mol Med. 9(1):61-77. PubMed
  18. Augé E, et al. 2017. Sci Rep. 7:41807. PubMed
  19. Kirouac L, et al. 2017. eNeuro. 10.1523/ENEURO.0149-16.2017. PubMed
  20. Willén K, et al. 2017. Mol Neurodegener. 10.1186/s13024-017-0203-y. PubMed
  21. Rocchi A, et al. 2017. PLoS Genet.. 10.1371/journal.pgen.1006962. PubMed
  22. Park J,et al. 2017. J Radiat Res.. 10.1093/jrr/rrx045. PubMed
  23. Zhi D, et al. 2017. Sci Rep. 10.1038/s41598-017-11628-9. PubMed
  24. Chen L, et al. 2017. Sci Rep. 10.1038/s41598-017-12484-3. PubMed
  25. Faraz A, et al. 2017. Antioxid Redox Signal. . 10.1089/ars.2016.6860. PubMed
  26. Casali BT, et al. 2018. J Neuroinflammation. 15:43. PubMed
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  29. Chaney A, et al. 2018. J Neurochem. 9:494. PubMed
  30. Datta M 2018. Immunity. 48:514. PubMed
  31. Ahmad F 2018. Scientific reports. 8:13119. PubMed
  32. Pan RY, et al. 2019. Sci Adv. 5:eaau6328. PubMed
  33. Chiang ACA, et al. 2018. Am J Pathol. 188:739. PubMed
  34. Hung COY, et al. 2018. Cell Rep. 25:3647. PubMed
  35. Kulas JA, et al. 2017. J Endocrinol. 235:49. PubMed
  36. Yang C, et al. 2017. Autophagy. :0. PubMed
  37. Bartolotti N, et al. 2018. J Alzheimers Dis. 66:1425. PubMed
  38. Sun J, et al. 2019. Nat Commun. 10:53. PubMed
  39. Limbocker R, et al. 2019. Nat Commun. 10:225. PubMed
  40. Leonetti M, et al. 2019. Sci Rep. 9:4591. PubMed
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  42. Poksay KS, et al. 2017. Front Pharmacol. 8:46. PubMed
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  46. Song M, et al. 2018. Genes (Basel). 9:. PubMed
  47. Dong ZZ, et al. 2018. Int J Ophthalmol. 11:747. PubMed
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  49. Sri S, et al. 2019. Acta Neuropathol Commun. 7:25. PubMed
  50. Feng T, et al. 2017. J Biol Chem. 292:1679. PubMed
  51. Garcia–Esparcia P, et al. 2017. Front Neurol. 8:89. PubMed
  52. Lin TW, et al. 2017. PLoS One. 12:e0174561. PubMed
  53. Amar F, et al. 2017. Sci Signal. 10:478. PubMed
  54. López–Arias B, et al. 2017. PLoS One. 12:e0177541. PubMed
  55. Finke JM, et al. 2017. Biochim Biophys Acta. 1861:2228. PubMed
  56. Hu X, et al. 2017. Front Neuroanat. 11:45. PubMed
  57. Kedia N, et al. 2017. Phys Chem Chem Phys. 19:18036. PubMed
  58. Li N, et al. 2017. Curr Alzheimer Res. 14:1109. PubMed
  59. O'Reilly MA, et al. 2017. Theranostics. 7:3573. PubMed
  60. Welty S, et al. 2018. J Biol Chem. 293:1353. PubMed
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  62. Ayaki T, et al. 2018. J Neuropathol Exp Neurol. 77:128. PubMed
  63. Lim CS, et al. 2018. Biosci Rep. 38:1. PubMed
  64. Söllvander S, et al. 2018. J Neuroinflammation. 15:98. PubMed
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Publication Library
RRID
AB_2715854 (BioLegend Cat. No. 803004)
AB_2564653 (BioLegend Cat. No. 803001)
AB_2564654 (BioLegend Cat. No. 803002)
AB_2564652 (BioLegend Cat. No. 803003)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, et al. 2012.  Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, et al.  2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 1-16 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 05-08-2018

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