Propidium Iodide Solution

Pricing & Availability
Regulatory Status
RUO
Other Names
PI
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
421301 2 mL 44€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Propidium iodide (PI) is a fluorescent dye that binds to DNA. When excited by 488nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.
Concentration
0.5 mg/ml
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protect from light. Caution: This solution contains hazardous material; handle with care.
Application

FC - Quality tested

Recommended Usage

The suggested use of this solution for viability staining is 10 µl per million cells in 0.5 ml/test, and incubate for 15 minutes at 4 °C before analysis. For Cell Cycle analysis, please see our Propidium Iodide Cell Cycle Staining Protocol. Caution: This solution is toxigenic and mutagenic; handle with care.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry.

Application References
  1. Vermes I, et al.1995. J. Immunol. Methods 184:39.
  2. Darzynkiewicz Z, et al. 1992. Cytometry 13(8):795.
  3. Douglas RS, et al. 1995. J. Immunol. Methods 188:219.
  4. Sakimoto I, et al. 2006. Cancer Research 66:2287.PubMed
  5. Liu H, et al. 2012. Int J Biochem Cell Biol. 45:408. PubMed
  6. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
  7. Ren Q, et al. 2013. PLoS One. 8:74732. PubMed
  8. Zhao W, et al. 2013. Clin Immunol. 149:119. PubMed
  9. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
  10. Ewald SE, et al. 2014. Infect Immun. 82:460. PubMed
  11. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
  12. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
Product Citations
  1. Liu H, et al. 2012. Int J Biochem Cell Biol. 45:408. PubMed
  2. Ewald S, et al. 2014. Infect Immun . 82:460. PubMed
  3. Guo H, et al. 2016. Sci Rep. 6:37559. PubMed
  4. Smith A, et al. 2017. Sci Rep. 7:41872. PubMed
  5. Li W, et al. 2019. Aging (Albany NY). 0.99375. PubMed
  6. Chan J, et al. 2017. Oncogene. 10.1038/onc.2017.319. PubMed
  7. Uroda T, et al. 2019. Mol Cell. 75:982. PubMed
  8. Lindqvist B, et al. 2022. PLoS Pathog. 18:e1010555. PubMed
  9. Neumann CKA, et al. 2020. Mol Metab. 34:136. PubMed
  10. Hamel Y, et al. 2021. Cell Rep Med. 2:100370. PubMed
  11. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  12. Leeth C, et al. 2021. Animals (Basel). 11:. PubMed
  13. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  14. McNeal AS, et al. 2021. Elife. 10:. PubMed
  15. Ren Q, et al. 2013. PLoS One. 8:74732. PubMed
  16. He Y, et al. 2020. Nat Commun. 11:1996. PubMed
  17. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  18. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  19. Yang Y, et al. 2021. Metabolism. 123:154863. PubMed
  20. Coray M, et al. 2022. Int J Mol Sci. 23:. PubMed
  21. Schrier S, et al. 2016. Sci Rep. 6:34942. PubMed
  22. O'Neill K, et al. 2016. Genes Dev. 30: 973-988. PubMed
  23. Sapanidou V, et al. 2015. Theriogenology. . PubMed
  24. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  25. Zeng H, et al. 2018. Cancer Cell. 34:56:00. PubMed
  26. Lei W, et al. 2020. Sci Rep. 10:7004. PubMed
  27. Wang Y, et al. 2019. Cell. 179:1144. PubMed
  28. Zhang M, et al. 2017. Oncol Lett. 3.069444444. PubMed
  29. Zhang W, et al. 2021. J Clin Invest. 131:. PubMed
  30. Ambrosi TH, et al. 2021. Elife. 10: . PubMed
  31. Feng L, et al. 2016. J Cell Sci. 129: 2202 - 2212. PubMed
  32. Lange N, et al. 2016. PLoS One. 11: 0160843. PubMed
  33. Jordan PM, et al. 2020. Cell Rep. 33:108247. PubMed
  34. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  35. Liu L, et al. 2019. Cell Prolif. :e12718. PubMed
  36. Zou PA, et al. 2021. Oncol Lett. 22:648. PubMed
  37. Masuda T, et al. 2022. iScience. 25:104781. PubMed
  38. Reighard SD, et al. 2020. Cell Rep Med. :1. PubMed
  39. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  40. Xu B, et al. 2016. Leukemia. 10.1038/leu.2016.297. PubMed
  41. Liu Y, et al. 2017. Acta Biomater. 10.1016/j.actbio.2017.08.021. PubMed
  42. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  43. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  44. Yang M, et al. 2021. Med Sci Monit. 27:e930083. PubMed
  45. Zhang X, et al. 2020. Eur J Med Chem. 112186:192. PubMed
  46. Mensen A, et al. 2015. Biol Blood Marrow Transplant. Available online 10 August 2015. PubMed
  47. Zhao W, et al. 2013. Clin Immunol. 149:119. PubMed
  48. Liu H, et al. 2020. Cell Death Dis. 0.767361111. PubMed
  49. Li L, et al. 2018. Oncol Lett. 4.184722222. PubMed
  50. Jung E, et al. 2017. J Neurosci. 37:6837. PubMed
  51. Basso-Ricci L, et al. 2017. Cytometry A. 10.1002/cyto.a.23148. PubMed
  52. Matsuda K, et al. 2021. Cell Rep Methods. 1:100122. PubMed
  53. Pang K, et al. 2022. Cell Death Dis. 13:169. PubMed
  54. Tang X, et al. 2021. Transl Cancer Res. 10:2120. PubMed
  55. Li Y, et al. 2016. Int J Lower Extrem Wounds. 10.1177/1534734616649485. PubMed
  56. Hu D, et al. 2020. Proc Natl Acad Sci U S A. 117:30639. PubMed
  57. Li Q, et al. 2020. Acta Biochim Biophys Sin (Shanghai). 52:1131. PubMed
  58. Moribe F, et al. 2021. PLoS One. 16:e0237554. PubMed
  59. Litzenburger UM, et al. 2017. Genome Biol. 18:15. PubMed
  60. Shanahan MT, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G668. PubMed
  61. Wang J, et al. 2022. J Clin Invest. :. PubMed
  62. Trudu M, et al. 2022. Front Oncol. 12:968208. PubMed
  63. L Chang, Y Li, D Kaplan 2016. Sci Rep. 6:36862. PubMed
  64. Perl AL, et al. 2019. J Biol Chem. 294:17043. PubMed
  65. Diaz Osterman CJ, et al. 2019. Elife. 8:e47327. PubMed
  66. Hosokawa T, et al. 2017. J Immunol. 10.4049/jimmunol.1700157. PubMed
  67. El-Hafeez A, et al. 2017. Cytotechnology. 10.1007/s10616-017-0124-1. PubMed
  68. Xue G, et al. 2021. Nat Biomed Eng. 5:1306. PubMed
  69. Lasrado N, et al. 2022. iScience. 25:103865. PubMed
  70. Tsou W, et al. 2014. J Biol Chem. 289:25750. PubMed
  71. Oubaha M, et al. 2016. Sci Transl Med. 8: 362ra144. PubMed
  72. Polk A, et al. 2016. Clin Cancer Res. 22(24):6118-6128. PubMed
  73. Harro CM, et al. 2020. J Clin Invest. . PubMed
  74. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  75. Suzuki S, et al. 2021. STAR Protocols. 2(3):100683. PubMed
  76. Vickman R, et al. 2016. Mol Cancer Res. 14: 776 - 786. PubMed
  77. El-Hafeez A, et al. 2017. Cytotechnology. 10.1007/s10616-017-0116-1. PubMed
  78. Xie M, et al. 2017. BMC Cancer . 10.1186/s12885-017-3291-8. PubMed
  79. Pal P, et al. 2021. J Med Chem. 64:14230. PubMed
  80. Hedemann N, et al. 2021. Cancers (Basel). 13:. PubMed
  81. Xie B, et al. 2021. J Immunol. 206:1515. PubMed
  82. M H, et al. 2016. Cell Transplant. 25: 811-27. PubMed
  83. Woo MS, et al. 2021. J Exp Med. :218. PubMed
  84. Huang K, et al. 2018. Biol Open. 7:bio029470. PubMed
  85. Wang H, et al. 2019. Nat Commun. 4.388194444. PubMed
  86. Matsubara T, et al. 2022. J Exp Med. 219:. PubMed
  87. Hughes K, et al. 2017. Open Bio. 10.1098/rsob.160155. PubMed
  88. Resch U, et al. 2016. MBio. 7: e00207-16. PubMed
  89. Bao W, et al. 2016. Sci Rep. 6:22579. PubMed
  90. Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed
  91. Yang X, et al. 2017. J Control Release. 10.1016/j.jconrel.2017.07.014. PubMed
  92. Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed
  93. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  94. Beggs K, et al. 2014. Toxicol Sci. 137:91. PubMed
  95. Wang C, et al. 2017. Drug Deliv. 10.1080/10717544.2017.1293195. PubMed
  96. Nilsri N, et al. 2021. Sci Rep. 5255:11. PubMed
  97. Bjørnstad R, et al. 2019. Mol Cancer Ther. 1.14375. PubMed
  98. Ghura H, et al. 2021. Neoplasia. 23:837. PubMed
  99. He Y, et al. 2020. J Hematol Oncol. 0.607638889. PubMed
  100. Widodo N, et al. 2022. F1000Res. 11:169. PubMed
  101. Bensberg M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  102. Namas R, et al. 2016. Lupus Sci Med. 3: e000148. PubMed
  103. Chen Y, et al. 2016. J Biol Chem. 291: 13216 - 13228. PubMed
  104. Deng BB, et al. 2020. Cell Biol Int. 44:1890. PubMed
  105. Siddhartha V, et al. 2017. Artif Cells Nanomed Biotechnol . 10.1080/21691401.2017.1313267. PubMed
  106. Viseshakul N, et al. 2017. Parasitol Res. 10.1007/s00436-017-5589-5. PubMed
  107. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  108. Sakimoto I, et al. 2006. Cancer Res. 66:2287. PubMed
  109. Goel S, et al. 2021. Nat Commun. 12:5325. PubMed
  110. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
  111. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  112. Mahmoudi M, et al. 2016. Sci Rep. 6:26960. PubMed
  113. Juel H, et al. 2013. PLoS One. 8:64619. PubMed
  114. Popa S, et al. 2020. Wellcome Open Res. 0.249305556. PubMed
  115. Vulin M, et al. 2022. Oncogene. :. PubMed

Antigen Details

Biology Area
Cell Biology, Cell Cycle/DNA Replication, Neuroscience
Gene ID
NA

Related FAQs

Why is washing not recommended after the addition 7-AAD or PI addition when assessing viability?

These dyes bind in equilibrium with DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out.

Go To Top Version: 2    Revision Date: 10-08-2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account