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Propidium iodide (PI) is a fluorescent dye that binds to DNA. When excited by 488nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.Product Details
- Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.
- 0.5 mg/ml
- Storage & Handling
- The solution should be stored undiluted between 2°C and 8°C, and protect from light. Caution: This solution contains hazardous material; handle with care.
FC - Quality tested
- Recommended Usage
The suggested use of this solution for viability staining is 10 µl per million cells in 0.5 ml/test, and incubate for 15 minutes at 4 °C before analysis. For Cell Cycle analysis, please see our Propidium Iodide Cell Cycle Staining Protocol. Caution: This solution is toxigenic and mutagenic; handle with care.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry.
- Application References
- Vermes I, et al.1995. J. Immunol. Methods 184:39.
- Darzynkiewicz Z, et al. 1992. Cytometry 13(8):795.
- Douglas RS, et al. 1995. J. Immunol. Methods 188:219.
- Sakimoto I, et al. 2006. Cancer Research 66:2287.PubMed
- Liu H, et al. 2012. Int J Biochem Cell Biol. 45:408. PubMed
- Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
- Ren Q, et al. 2013. PLoS One. 8:74732. PubMed
- Zhao W, et al. 2013. Clin Immunol. 149:119. PubMed
- Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
- Ewald SE, et al. 2014. Infect Immun. 82:460. PubMed
- Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
- Kong S, et al. 2014. J Immunol. 193:5515. PubMed
- Product Citations
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Neuroscience
- Gene ID
- Why is washing not recommended after the addition 7-AAD or PI addition when assessing viability?
These dyes bind in equilibrium with DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out.