PE/Cyanine7 anti-human CD133 Antibody

Pricing & Availability
clone 7 (See other available formats)
Regulatory Status
Other Names
Prominin-1, AC133 Antigen, PROM1, PROML1
Mouse IgG1, κ
Ave. Rating
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Product Citations
Human PBMCs were stained with anti-CD34 FITC and PE/Cyanine7 CD133 (clone 7)(top) or PE/Cyanine7 mouse IgG1, ? isotype control (bottom). Data shown was gated on the lymphocyte population.
  • clone7_PECy7_CD133_Antibody_1_051717
    Human PBMCs were stained with anti-CD34 FITC and PE/Cyanine7 CD133 (clone 7)(top) or PE/Cyanine7 mouse IgG1, ? isotype control (bottom). Data shown was gated on the lymphocyte population.
  • clone7_PECy7_CD133_Antibody_2_051717
Compare all formats See PE/Cyanine7 spectral data
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372809 25 tests 128€
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372810 100 tests 296€
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CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant partial human CD133 protein.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone can block the staining of AC133 clone in flow cytometry.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Swaminathan SK, et al. 2010. J. Immunol. Methods 361:110.
Product Citations
  1. Nacarelli T, et al. 2020. Cancer Res. 80:890. PubMed
  2. Ruivo CF, et al. 2022. Gut. . PubMed
  3. Sasabe E, et al. 2022. Cancer Sci. 113:609. PubMed
  4. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  5. Zhai Y, et al. 2019. Oncol Rep. 41:1817. PubMed
AB_2686967 (BioLegend Cat. No. 372809)
AB_2686968 (BioLegend Cat. No. 372810)

Antigen Details

120 kD pentaspan transmembrane glycoprotein.

Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells

Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References
  1. Yin AH, et al. 1997. Blood. 90:5002.
  2. Miraglia S, et al. 1997. Blood. 90:5013.
  3. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25. 
Gene ID
8842 View all products for this Gene ID
View information about CD133 on

Related FAQs

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Go To Top Version: 0    Revision Date: 05-18-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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