MojoSort™ Human CD3 T Cell Isolation Kit

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PBMCs were prepared to isolate CD3+ T cells using the MojoSort™ Human CD3 T Cell Isolation Kit. Cells were stained with CD3ε (UCHT1) APC.
  • Human_CD3T_Isolation_Kit_1_091115
    PBMCs were prepared to isolate CD3+ T cells using the MojoSort™ Human CD3 T Cell Isolation Kit. Cells were stained with CD3ε (UCHT1) APC.
  • Human_CD3T_Isolation_Kit_2_091115
Cat # Size Price Quantity Check Availability Save
480021 10 tests 159€
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480022 100 tests 476€
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480131 200 tests 583€
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Non CD3+ T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched CD3+ T cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480021:

  • 100 µl Biotin-Antibody Cocktail
  • 100 µl Streptavidin Nanobeads


For Cat# 480022

  • 1 ml Biotin-Antibody Cocktail
  • 1 ml Streptavidin Nanobeads


For Cat# 480131

  • 2 vials of 1 ml Biotin-Antibody Cocktail
  • 2 vials of 1 ml Streptavidin Nanobeads

Product Details

Verified Reactivity
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of antibody cocktail for 1x107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer.

For this kit, incubation with antibody cocktail and Streptavidin Nanoparticles can be done for 5 to 15 minutes, without loss in purity and yield.

Application Notes

The expected purity is >95% CD3+ cells post-separation of human peripheral blood mononuclear cells from healthy donors, analyzed by flow cytometry

This kit is designed for the isolation of untouched CD3+ T cells from peripheral blood mononuclear cells (PBMCs).

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

Antibody or cocktail dilution to use in column: 2.5X
Nanobead dilution to use in columns: 4X

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Watch a Scientific Poster video of this product.

Product Citations
  1. Li W, et al. 2021. Front Pharmacol. 12:724306. PubMed
  2. Rennier K, et al. 2020. Clin Cancer Res. 26:5019. PubMed
  3. Feng Y, et al. 2022. Life (Basel). 12:. PubMed

Antigen Details

Biology Area
Molecular Family
CD Molecules, TCRs
Gene ID
View information about CD3 on

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 2    Revision Date: 01-26-2022

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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