FITC anti-mouse CD335 (NKp46) Antibody

Pricing & Availability
Clone
29A1.4 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Rat IgG2a, κ
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Product Citations
publications
29A1.4_FITC_1_060310
C57BL/6 splenocytes stained with NK1.1 (PK136) APC and 29A1.4 FITC
  • 29A1.4_FITC_1_060310
    C57BL/6 splenocytes stained with NK1.1 (PK136) APC and 29A1.4 FITC
  • 29A1.4_FITC_2_060310
    C57BL/6 splenocytes stained with NK1.1 (PK136) APC and rat IgG2a, κ FITC isotype control
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137605 25 µg 76€
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137606 100 µg 204€
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Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
NKP46-IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.

Application References
  1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
  2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
  3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations
  1. Calabrese DR, et al. 2020. J Clin Invest. . PubMed
  2. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  3. Saha S, et al. 2023. Sci Rep. 13:4609. PubMed
  4. Chang D, et al. 2023. Front Immunol. 14:1105145. PubMed
  5. Pelletier A, et al. 2023. EMBO Rep. 24:e56156. PubMed
  6. Rabia E, et al. 2023. Front Immunol. 14:1168444. PubMed
  7. Zhang Z, et al. 2023. Aging Dis. 14:966. PubMed
  8. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  9. Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed
  10. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  11. Shapiro MR, et al. 2020. Front Immunol. 1.972222222. PubMed
  12. Piranlioglu R, et al. 2019. Nat Commun. 10:1430. PubMed
  13. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  14. Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed
  15. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  16. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  17. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  18. Li J, et al. 2018. Immunity. 49:178. PubMed
  19. Marcus A et al. 2018. Immunity. 49(4):754-763 . PubMed
  20. Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed
  21. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  22. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
  23. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  24. Kawamura A, et al. 2016. Sci Rep. 6:19464. PubMed
  25. Martin MD, et al. 2020. Cell Rep. 31:107508. PubMed
  26. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  27. Bhatt D, et al. 2021. J Exp Med. 218:. PubMed
  28. Lam KC, et al. 2021. Cell. 184:5338. PubMed
  29. Natale CA, et al. 2018. Elife. 7. PubMed
  30. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  31. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  32. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  33. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
RRID
AB_2149150 (BioLegend Cat. No. 137605)
AB_2298210 (BioLegend Cat. No. 137606)

Antigen Details

Structure
Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution

Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells

Function
NK cells activation
Ligand/Receptor
Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.

Gene ID
17086 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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