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Cell-Vive™ CD RBC Lysis Buffer (10X), GMP

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Other Names
Red blood cell lysis buffer, lysis buffer, CD buffer, RBC Buffer
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Cell-Vive_CD-RBC-Lysis-Buffer_GMP_032723
Human peripheral whole blood was analyzed by flow cytometry before the lysis of red blood cells (RBC) (A) or after the treatment with 1X solution of Cell-Vive™ CD RBC Lysis Buffer 10X, GMP (B).
  • Cell-Vive_CD-RBC-Lysis-Buffer_GMP_032723
    Human peripheral whole blood was analyzed by flow cytometry before the lysis of red blood cells (RBC) (A) or after the treatment with 1X solution of Cell-Vive™ CD RBC Lysis Buffer 10X, GMP (B).
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420514 200 mL 200€
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Description

Cell-Vive™ CD RBC Lysis Buffer 10X, GMP is a chemically-defined buffer designed to insure optimal lysis of human red blood cells (RBC) with minimal effects on leukocytes. This product is formulated without animal-derived components or preservatives, contains low levels of endotoxin (< 1EU/mL), and is tested negative for mycoplasma and microbial growths. Cell-Vive™ CD RBC lysis Buffer 10X, GMP is a 10X solution that should be diluted to 1X before use. This GMP product is for research and further ex vivo bioprocessing use only.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Chemically-Defined, no preservatives. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water
Preparation
Chemically-Defined, no preservatives. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water. Warm the 1X solution to room temperature before use.
Storage & Handling
Store between 2°C-8°C
Recommended Usage

Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water. 2 ml of 1X Cell-Vive™ CD RBC Lysis Buffer, GMP is enough to lyse up to 100 μl of whole blood.

Application Notes

Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is recommended for the lysis of human red blood cells. This buffer has a chemically-defined formulation without animal-derived components, it contains low levels of endotoxin (< 1EU/mL), and it is tested negative for mycoplasma and microbial growths. Use Cell-Vive™ CD RBC Lysis Buffer (10X), GMP for highly sensitive assays.

 

Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X formulation that contains no preservatives, handle under aseptic conditions as needed.

Additional Product Notes

Human Red Blood Cells lysis protocol

  1. Dilute the Cell-Vive™ CD RBC Lysis Buffer (10X), GMP to 1X working concentration with deionized water. Warm the 1X solution to room temperature before use.
  2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 μl of human whole blood.
  3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature for 10-15 minutes.
  4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing the pellet.
  5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell-Vive™ CD Cell Staining Buffer, GMP Cat. No. 420508), and wash 1X.
  6. Resuspend and proceed with further procedures.

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 04-07-2023

For research and further manufacturing use. Not for diagnostic or therapeutic procedures. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to reverse engineer functionally similar materials without written approval of BioLegend. By use of these products you accept the terms and conditions of any and all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research and further manufacturing use only and not intended for human or animal diagnostic or therapeutic use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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