- RTK2071 (See other available formats)
- Regulatory Status
- Rat IgG1, κ
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- Product Citations
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The isotype of RTK2071 immunoglobulin is rat IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.Product Details
- Antibody Type
- Host Species
- Trinitrophenol + KLH
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
- µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
ICFC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The RTK2071 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400414) as negative control1. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400432) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
- Application References
- Product Citations
- Gene ID
Related Pages & Pathways
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC Rat IgG1, κ Isotype Ctrl
Biotin Rat IgG1, κ Isotype Ctrl
FITC Rat IgG1, κ Isotype Ctrl
PE Rat IgG1, κ Isotype Ctrl
PE/Cyanine5 Rat IgG1, κ Isotype Ctrl
Purified Rat IgG1, κ Isotype Ctrl
PE/Cyanine7 Rat IgG1, κ Isotype Ctrl
Alexa Fluor® 647 Rat IgG1, κ Isotype Ctrl
Alexa Fluor® 488 Rat IgG1, κ Isotype Ctrl
Pacific Blue™ Rat IgG1, κ Isotype Ctrl
Alexa Fluor® 700 Rat IgG1, κ Isotype Ctrl
APC/Cyanine7 Rat IgG1, κ Isotype Ctrl
PerCP Rat IgG1, κ Isotype Ctrl
PerCP/Cyanine5.5 Rat IgG1, κ Isotype Ctrl
Brilliant Violet 421™ Rat IgG1, κ Isotype Ctrl
Ultra-LEAF™ Purified Rat IgG1, κ Isotype Ctrl
Brilliant Violet 605™ Rat IgG1, κ Isotype Ctrl
Brilliant Violet 510™ Rat IgG1, κ Isotype Ctrl
Brilliant Violet 650™ Rat IgG1, κ Isotype Ctrl
Brilliant Violet 711™ Rat IgG1, κ Isotype Ctrl
Brilliant Violet 785™ Rat IgG1, κ Isotype Ctrl
PE/Dazzle™ 594 Rat IgG1, κ Isotype Ctrl
Alexa Fluor® 594 Rat IgG1, κ Isotype Ctrl
GoInVivo™ Purified Rat IgG1, κ Isotype Ctrl
APC/Fire™ 750 Rat IgG1, κ Isotype Ctrl
TotalSeq™-A0236 Rat IgG1, κ Isotype Ctrl
KIRAVIA Blue 520™ Rat IgG1, κ Isotype Ctrl
TotalSeq™-B0236 Rat IgG1, κ Isotype Ctrl
TotalSeq™-C0236 Rat IgG1, κ Isotype Ctrl
Spark YG™ 581 Rat IgG1, κ Isotype Ctrl
Spark YG™ 593 Rat IgG1, κ Isotype Ctrl