Brilliant Violet 605™ anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
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Product Citations
publications
N418_BV605_052112
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 605™.
  • N418_BV605_052112
    C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 605™.
See Brilliant Violet 605™ spectral data
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117333 125 µL 156,00€
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117334 50 µg 208,00€
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).

Application References
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
Product Citations
  1. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  2. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  3. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  4. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  5. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  6. Engblom C, et al. 2017. Science. 358:6367. PubMed
  7. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  8. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  9. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  10. Acker KP, et al. 2019. iScience. 19:281. PubMed
  11. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
  12. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  13. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  14. Wang ECE, et al. 2019. Cell Stem Cell. 24:654. PubMed
  15. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  16. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  17. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  18. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  19. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  20. Li J, et al. 2018. Immunity. 49:178. PubMed
  21. Pundir P, et al. 2020. Cell Host & Microbe. 26(1):114-122. PubMed
  22. Guo Y, et al. 2020. Cell Host & Microbe. 28(1):104-116.e4.. PubMed
  23. McGinley AM, et al. 2020. Immunity. 52(2):342-356. PubMed
  24. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  25. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  26. Pennock ND, et al. 2018. J Immunother Cancer. 0.318055556. PubMed
  27. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  28. Allman W, et al. 2015. Proc Natl Acad Sci U S A. 112: 4094 - 4103. PubMed
  29. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  30. Xu AQ, et al. 2020. Elife. 9:00. PubMed
  31. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  32. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  33. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  34. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  35. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  36. Ehrens A, et al. 2021. Cell Reports. 34(2):108621. PubMed
  37. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  38. Dewi IMW, et al. 2021. Cell Reports Medicine. 2(5):100289. PubMed
  39. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  40. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  41. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
  42. Bruand M, et al. 2021. Cell Reports. 36(3):109412. PubMed
  43. Magod P, et al. 2021. Cell Reports. 36(5):109480. PubMed
  44. Park JG, et al. 2021. iScience. 24(9):102941. PubMed
  45. Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
  46. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  47. Li J, et al. 2020. Cancer Discov. . PubMed
  48. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
RRID
AB_11204262 (BioLegend Cat. No. 117333)
AB_2562415 (BioLegend Cat. No. 117334)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10-21-2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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