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Streptavidin binds to biotin with high affinity. Streptavidin-APC/Cyanine7 is useful for detecting biotinylated antibodies. The excitation of APC/Cyanine7 by 600-635 nm laser light induces a fluorescence emission maximum of 780 nm.Product Details
- Human, Mouse, Rat, All Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Streptavidin is conjugated with APC/Cyanine7 under optimal conditions.
- 0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
- Storage & Handling
- The Streptavidin APC/Cyanine7 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC, ICFC - Quality tested
- Recommended Usage
Each lot of this Streptavidin APC/Cyanine7 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Cyanine7 conjugation. For immunofluorescent staining, the recommended use of this reagent is =0.06 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Streptavidin-Allophycocyanin/Cyanine7 (APC/Cyanine7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC/Cyanine7 is 350 kD and Streptavidin alone is 52 kD.
- Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
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- Peterson BC, et al. 2014. J Leukoc Biol. 95:809. PubMed
- Barbera M, et al. 2015. Gut. 64:11. PubMed
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- Product Citations