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- Clone
- N150/21 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DOC2BL, Double C2-like domain-containing protein beta, doc2-beta, double C2, beta
- Previously
-
Covance Catalog# MMS-5250
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-Doc2b antibody (clone N150/21). Lane 1, 5: Molecular weight marker; Lane 2, 6: 50 µg of human brain lysate; Lane 3, 7: 50 µg of rat brain lysate; Lane 4, 8: 50 µg of mouse brain lysate. The blots were incubated with 1 µg/mL of clone N150/21 (left) or mouse IgG1 isotype control (right) overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The additional bands observed in mouse brain lysates correspond to non-specific bands.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 833201 | 100 µL | 132€ | ||||
Double C2-like domain-containing protein beta (DOC2b) is a SNARE-related protein that binds to syntaxin-4 in a calcium-dependent manner to regulate exocytosis. DOC2b is stimulated by insulin to mediate glucose intake in the pancreas and can modulate release of neurotransmitters in the CNS.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-216 (N-terminus) of rat Doc2b.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB).
This antibody does not exhibit cross-reactivity to Doc2a. - RRID
-
AB_2564964 (BioLegend Cat. No. 833201)
Antigen Details
- Structure
- Expected MW: 45 kD
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Gene ID
- 81820 View all products for this Gene ID
- UniProt
- View information about Doc2b on UniProt.org
Related FAQs
Other Formats
View All Doc2b Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Doc2b | N150/21 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Doc2b
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