Purified anti-Doc2b Antibody (Previously Covance catalog# MMS-5250)

Pricing & Availability
Clone
N150/21 (See other available formats)
Regulatory Status
RUO
Other Names
DOC2BL, Double C2-like domain-containing protein beta, doc2-beta, double C2, beta
Previously
Covance Catalog# MMS-5250
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N150slash21_Purified_Doc2b_Antibody_081718
Western blot of purified anti-Doc2b antibody (clone N150/21). Lane 1, 5: Molecular weight marker; Lane 2, 6: 50 µg of human brain lysate; Lane 3, 7: 50 µg of rat brain lysate; Lane 4, 8: 50 µg of mouse brain lysate. The blots were incubated with 1 µg/mL of clone N150/21 (left) or mouse IgG1 isotype control (right) overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The additional bands observed in mouse brain lysates correspond to non-specific bands.
  • N150slash21_Purified_Doc2b_Antibody_081718
    Western blot of purified anti-Doc2b antibody (clone N150/21). Lane 1, 5: Molecular weight marker; Lane 2, 6: 50 µg of human brain lysate; Lane 3, 7: 50 µg of rat brain lysate; Lane 4, 8: 50 µg of mouse brain lysate. The blots were incubated with 1 µg/mL of clone N150/21 (left) or mouse IgG1 isotype control (right) overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The additional bands observed in mouse brain lysates correspond to non-specific bands.
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833201 100 µL 132€
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Description

Double C2-like domain-containing protein beta (DOC2b) is a SNARE-related protein that binds to syntaxin-4 in a calcium-dependent manner to regulate exocytosis. DOC2b is stimulated by insulin to mediate glucose intake in the pancreas and can modulate release of neurotransmitters in the CNS.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-216 (N-terminus) of rat Doc2b.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

This antibody does not exhibit cross-reactivity to Doc2a.

RRID
AB_2564964 (BioLegend Cat. No. 833201)

Antigen Details

Structure
Expected MW: 45 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
81820 View all products for this Gene ID
UniProt
View information about Doc2b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06/13/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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  • Purified anti-Doc2b

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