LEGEND MAX™ Human Granulysin ELISA Kit

Pricing & Availability
Other Names
Granulysin Pre-coated ELISA Kit
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Product Citations
publications
Granulysin_Human_052511
  • Granulysin_Human_052511
Cat # Size Price Quantity Save
438007 1 Pre-coated Plate 316€
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Description

Granulysin is a cytolytic protein and present in granules of human natural killer (NK) cells and activated cytotoxix T lymphocytes (CTLs). Granulysin is synthesized as a 15 kD precursor, which is then cleaved at both the amino- and carboxy-termini into a 9 kD activated form. The 9 kD granulysin is released into the intercellular space between target and effector cells via a granule exocytosis pathway.

The 9 kD granulysin is a chemoattractant for T lymphocytes, monocytes, and other inflammatory cells and activates the expression of a number of cytokines, including RANTES, MCP-1, MCP-3, MIP-1a, IL-10, IL-1, IL-6 and IFNa.

High serum levels of granulysin is associated with cytotoxic T-cell and NK cell activity and may thus represent a soluble biomarker for host-cellular immune responses. Serum granulysin has also been proposed as a biomarker for the Th1/Th2 balance and at the cellular level, granulysin may serve as a favorable prognostic marker for certain types of cancer.

The LEGEND MAX™ Human Granulysin ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with 96-well strip plates that are pre-coated with a capture antibody.

This assay detects both 9 kD and 15 kD form of human granulysin from cell culture supernatant, serum, plasma, or other body fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human Granulysin Pre-coated 96-well Strip Microplate
  • Human Granulysin Dectection Antibody
  • Human Granulysin Standard
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Application References
  1. Ono S, et al. 2014. J Dermatol Sci. 73:74. PubMed
Product Citations
  1. Ono S, et al. 2014. J Dermatol Sci. 73:74. PubMed
  2. Wu CH, et al. 2019. J Extracell Vesicles. 8:1588538. PubMed
Publication Library
Sensitivity
0.3 ng/mL
Standard Range
0.63-40 ng/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
NK cells, cytolytic T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
10578 View all products for this Gene ID
UniProt
View information about Granulysin on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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