Product: APC anti-mouse Ly-6G |
Catalog No.: 127613 |
Patrick Murphy, Graduate Student University of Rochester |
Ly6G-APC | |||
Overall: | Product Quality: | Ease of Use: | |
A bright Ly6G option - clearly identifies neutrophils. |
Experimental Design |
Application: | Flow Cytometry |
Cells used: | Inflamed peritoneal lavage cells (Murine) |
Brief Protocol: | Fc block with 1:100 anti-CD16/32 on ice for 10 minutes. Stain with 1:200 Ly6g-APC and 1:400 CD11b-PE on ice for 30 mins. Wash once and resuspend in 300ul FACS buffer Analyze on FACS Calibur. |
Results Summary: | Clear identification of neutrophils by CD11bint/Ly6ghi. |
Additional Notes: |
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