Product: PE anti-mouse CD365 (Tim-1) |
Catalog No.: 119506 |
Qing Ding, Research Scientist University of Pittsburgh |
The Signal is Weak, More Color Labeled Antibody Please | |||
Overall: | Product Quality: | Ease of Use: | |
More color please. |
Experimental Design |
Application: | TIM-1 expression on mice B cells. |
Cells used: | Mice spleen cells |
Brief Protocol: | Surface staining, mice spleen cells stained with CD19 Alexa Fluor® 647 & TIM-1-PE for 45min-60 min on ice. |
Results Summary: | OK, not perfect. |
Additional Notes: | ~3% CD19+B cells are TIM-1+ with regular LSR, while ~10% CD19+B cells express TIM-1 with LSR Fortes, Green Laser(532nm). |
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