Product: FITC anti-mouse/human CD44 |
Catalog No.: 103022 |
Joy Hsu, Specialist UCSF |
FITC anti-mouse/human CD44 Antibody | |||
Overall: | Product Quality: | Ease of Use: | |
Staining works, not a great tumour marker. |
Experimental Design |
Application: | flow cytometry |
Cells used: | human tumour infiltrate |
Brief Protocol: | Cell suspension was plated in 96-well plate at 2-4 million cells/ well. Antibody was added in proper flow buffer at 2:100 for 30 minutes in the dark at 4 degrees Celsius. Used in conjunction with anti-human CD45, CD90, EPCAM in order to try to isolate tumour cells versus immune cells. |
Results Summary: | Staining worked, but is not a good marker indicative of tumour cells. EPCAM is a better differentiating marker. |
Additional Notes: |
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