Application: |
Flow cytometry |
Cells used: |
Mouse macrophages, monocytes, and epithelial cells |
Brief Protocol: |
I followed a standard flow cytometry staining protocol: Purify mouse bronchoalveolar lavage (spin and wash with 1X PAB buffer). Stain with panel, including BioLegend PE anti-mouse CD192 (CCR2). Fix, wash. |
Results Summary: |
Clear CCR2 + and - populations, easy differentiation of monocyte and epithelial cell subpopulations. |
Additional Notes: |
Excellent antibody, excellent addition to a BAL cell panel! |
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