Total lysates from HeLa (Lane 1), 5nM (Lane 2), and 20nM (Lane 3) TSC2 siRNA treated HeLa cells were probed with purified anti-TSC2 antibody (clone W16104A, Cat. No. 697701). Direct-Blot™ HRP anti-β-actin Antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control. M is MW ladder. M* is higher exposure of the ladder. | Total lysates from 293T (Lane 1) and 293T/HDAC2 CRISPR/Cas9 treated cells (Lane 2) were probed with purified anti-HDAC2 antibody (clone 13G8C67, Cat. No. 680102). Anti-GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control. Lane M: MW ladder, M* indicates longer exposure. | Total lysates from HeLa (Lane 1) and CRISPR/Cas9 treated HeLa cells (Lane 2) were probed with purified anti-AKT1 antibody (clone O94E10, Cat. No. 680302). Anti-GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control. Lane M: MW ladder. |