Purified anti-BRST-3 Antibody

Pricing & Availability
Clone
B72.3 (See other available formats)
Regulatory Status
RUO
Other Names
BRST3
Isotype
Mouse IgG1, κ
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Product Citations
publications
B72point3_PURE_BRST3_Antibody_1_081718
IHC staining of purified anti-BRST-3 antibody (clone B72.3) on formalin-fixed paraffin-embedded human breast tumor tissue. The tissue was incubated with 4 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B72point3_PURE_BRST3_Antibody_1_081718
    IHC staining of purified anti-BRST-3 antibody (clone B72.3) on formalin-fixed paraffin-embedded human breast tumor tissue. The tissue was incubated with 4 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B72point3_PURE_BRST3_Antibody_2_081718
    IHC staining of purified anti-BRST-3 antibody (clone B72.3) on formalin-fixed paraffin-embedded human breast tumor tissue. The tissue was incubated with 4 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Save
915206 100 µg ¥50,380
Description

BRST-3, also known as TAG-72 (Tumor Associated Glycoprotein-72), a high molecular weight (220-400 kD) glycoprotein complex, is expressed by a wide variety of human adenocarcinomas.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against a membrane-enriched fraction of a breast carcinoma metastatic to the liver.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

B72.3 is reactive with a broad spectrum of epithelial malignancies in human, including invasive ductal carcinoma, lung carcinomas, ovarian, gastric and colonic adenocarcinomas. It appears to be non-reactive or weakly reactive to most normal tissues.

Application References

(PubMed link indicates BioLegend citation)
  1. Liu G, et al. 2008. Eur. J. Nucl. Med. Mol. Imaging 35(2):272.
  2. Lyda MH, et al. 2000. Hum. Pathol. 31(8):980.
  3. Clemo FA, et al. 1995. Vet. Pathol. 32:147. (IHC)
  4. Colcher D, et al. 1981. Proc. Natl. Acad. Sci. USA 78:3199.
RRID
AB_2616965 (BioLegend Cat. No. 915206)

Antigen Details

Biology Area
Cancer Biomarkers, Cell Biology
Gene ID
NA
UniProt
View information about BRST-3 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 08/17/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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