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Ultra-LEAF™ Purified anti-human Galectin-9 Antibody

Pricing & Availability
Clone
W21100D (See other available formats)
Regulatory Status
RUO
Other Names
HUAT, Lectin, galactoside-binding, soluble, 9 (LGALS9), LGALS9A
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W21100D_ULEAF_Galectin-9_Block_042229
Recombinant Human TIM-3-Fc chimera (Cat. No 759904) (black circles) binds to immobilized recombinant Human Galectin-9 (E. coli expressed) (Cat. No. 754806). Ultra-LEAF™ purified anti-human Galectin-9 (clone W21100D) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have effect. This antibody blocks the binding of 2 µg/mL human TIM-3-Fc chimera to 5 µg/mL immobilized recombinant human Galectin-9 (E. coli expressed). ND50 range: 0.5 - 3 µg/mL
  • W21100D_ULEAF_Galectin-9_Block_042229
    Recombinant Human TIM-3-Fc chimera (Cat. No 759904) (black circles) binds to immobilized recombinant Human Galectin-9 (E. coli expressed) (Cat. No. 754806). Ultra-LEAF™ purified anti-human Galectin-9 (clone W21100D) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have effect. This antibody blocks the binding of 2 µg/mL human TIM-3-Fc chimera to 5 µg/mL immobilized recombinant human Galectin-9 (E. coli expressed). ND50 range: 0.5 - 3 µg/mL
Cat # Size Price Save
637053 100 µg ¥75,530
637054 1 mg ¥195,880
Description

Galectins constitute a family of conserved animal lectins, which are characterized by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and sequence similarities in the carbohydrate recognition domain (CRD). In vertebrates, the Galectin family comprises 15 members. Galectins have been classified into three types: a) Prototype monomeric Galectins with a single polypeptide chain that dimerize (Galectin-1, -2, -5, -7, -10, -11, -13 -14, and -15); b) Tandem repeat galectins composed of a single polypeptide chain with two CRDs, joined by a linker peptide (Galectin-4, -6, -8, -9, and -12); and c) Chimeric galectin, thus far containing only Galectin-3, which consists of one C-terminal CRD linked to an N-terminal domain. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 functions in effector and regulatory phases of the immune response. During inflammation, the production of Galectin-9 in vivo generates a microenvironment that limits effector T cell responses. Galectin-9 can negatively regulate the Th1 immune response by engaging TIM-3 on T cells, which leads to induction of intracellular Ca2+ influx and activation of apoptosis pathways. In addition, Galectin-9 has been shown to function as a urate transporter in the kidney. Galectin-9 has been identified as a possible prognostic marker in breast cancer, melanoma, and oral squamous cell carcinomas.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human Galectin-9
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 2 µg/mL recombinant human TIM-3-Fc chimera (Cat. No 759904) to immobilized 5 µg/mL recombinant human Galectin-9 (E. coli expressed) (Cat. No. 754806). ND50 = 0.5 – 3 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Homodimer
Distribution

Galectin-9 is expressed by lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal cells, and epithelial cells.

Function
Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells. IFN-γ induces galectin-9 expression in fibroblasts, endothelial, and Kupffer cells. IL-1β and IL-5 induces it in astrocytes and eosinophils, respectively.
Interaction
Extracellular matrix
Ligand/Receptor
Galectin-9 binds to glycans featuring galactose and its derivatives: CD44 and TIM-3.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References
  1. Toscano MA, et al. 2009. Nat Immunol Rev. 9:338-52.
  2. Hadari YR, et al. 2000. J Cell Sci. 113:2385-52.
  3. Tsuboi Y, et al. 2007. Clin Immunol. 124:221-33.
  4. Zhu C, et al. 2005. Nat Immunol. 6:1245-52.
  5. Wu C, et al. 2014. Immunity. 41:270-82.
  6. Graessler J, et al. 2000. Adv Exp Med Biol. 486:179-83.
  7. Bacigalupo ML, et al. 2013. World J Gastroenterol. 47:8831-49.
Gene ID
3965 View all products for this Gene ID
UniProt
View information about Galectin-9 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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