- 1D11 (See other available formats)
- Regulatory Status
- Other Names
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NK cells, CD8+ T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
IP, FA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
(PubMed link indicates BioLegend citation)
- Wu J, et al. 1999. Science 285:730.
- Wu J, et al. 2000. J. Exp. Med. 192:1059.
- Groh V, et al. 2001. Nature Immunol. 2:255.
- Wu J, et al. 2002. J. Immunol. 169:1236.
- Roberts A, et al. 2001. J. Immunol. 167:5527.
- Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
- Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
- Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
- Watanbe M, et al. 2014. Int Immunol. PubMed
- Product Citations
AB_2810480 (BioLegend Cat. No. 320813)
AB_2561488 (BioLegend Cat. No. 320814)
- C-type lectin
NK cells, γ/δ T cells, CD8+ T cells
- Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
- MICA, MICB, UL16-binding proteins (ULBPs)
- Cell Type
- NK cells, T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Jamieson AM, et al. 2002. Immunity 17:19.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.
7. Maasho K, et al. 2005. J. Immunol. 174:4480.
8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
- Gene ID
- 22914 View all products for this Gene ID
- View information about CD314 on UniProt.org
Related Pages & Pathways
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other FormatsView All CD314 Reagents Request Custom Conjugation
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD314 (NKG2D)
Biotin anti-human CD314 (NKG2D)
PE anti-human CD314 (NKG2D)
APC anti-human CD314 (NKG2D)
PE/Cyanine7 anti-human CD314 (NKG2D)
Ultra-LEAF™ Purified anti-human CD314 (NKG2D)
Brilliant Violet 510™ anti-human CD314 (NKG2D)
PerCP/Cyanine5.5 anti-human CD314 (NKG2D)
FITC anti-human CD314 (NKG2D)
Brilliant Violet 421™ anti-human CD314 (NKG2D)
APC/Cyanine7 anti-human CD314 (NKG2D)
Alexa Fluor® 647 anti-human CD314 (NKG2D)
PE/Dazzle™ 594 anti-human CD314 (NKG2D)
Brilliant Violet 785™ anti-human CD314 (NKG2D)
Brilliant Violet 605™ anti-human CD314 (NKG2D)
APC/Fire™ 750 anti-human CD314 (NKG2D)
TotalSeq™-A0165 anti-human CD314 (NKG2D)
TotalSeq™-C0165 anti-human CD314 (NKG2D)
TotalSeq™-B0165 anti-human CD314 (NKG2D)
Alexa Fluor® 660 anti-human CD314 (NKG2D) Antibody
PE/Cyanine5 anti-human CD314 (NKG2D)
TotalSeq™-D0165 anti-human CD314 (NKG2D)