Purified anti-Rad23B Antibody

Pricing & Availability
Clone
11A2SC (See other available formats)
Other Names
RAD23 homolog B (S. cerevisiae), RAD23, yeast homolog of B, UV excision repair protein RAD23 homolog B, HHR23B
Isotype
Mouse IgG1, λ
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11A2SC_Purified_Rad23B_Antibody_2_071916
Total lysates (15 µg protein) from 293T and 293T/Rad23B CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-Rad23B antibody, clone 11A2SC (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 11A2SC_Purified_Rad23B_Antibody_2_071916
    Total lysates (15 µg protein) from 293T and 293T/Rad23B CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-Rad23B antibody, clone 11A2SC (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 11A2SC_Purified_Rad23B_Antibody_WB_071916
    Total lysates (15 µg protein) from THP-1, HeLa, Raw264.7 and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2500 (0.2 µg/ml) purified anti- Rad23B antibody , clone 11A2SC (upper). Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M is the MW ladder.
Cat # Size Price Save
654201 25 µg ¥25,000
Description

Yeast Rad23, originally identified as a DNA repair protein, has been proposed to participate in other cellular functions, i.e., the proteasome-degradation pathway, the process of spindle pole body duplication, and as a component of the anaphase checkpoint. Two human homologs of yeast Rad23 (Rad23A and Rad23B) exhibit high sequence homology with yeast Rad23 and also have been shown to be involved in DNA repair and proteasome-dependent degradation.

Rad23B is involved in global genome nucleotide excision repair (GG-NER) by acting as a component of the XPC complex. The XPC complex recognizes a wide spectrum of damaged DNA, characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles, and single-stranded overhangs.

The Rad23 family provides an essential connection between the 26S proteasome and ubiquitinated proteins. It can bind simultaneously to the 26S proteasome and to polyubiquitinated substrates to deliver ubiquitinated proteins to the proteasome. It may play a role in endoplasmatic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and by delivering deglycosylated proteins to the proteasome. The interactions of Rad23B, Atx3, and valosin-containing protein (VCP) are dynamic and modulated by proteotoxic stresses. Recent studies also show that Rad23B is a candidate cancer biomarker identified in a genome-wide loss-of-function screen, which influences sensitivity to HDAC inhibitors.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human Rad23B protein.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
Preparation
Protein G affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Rad23B has two isoforms, and the predicted Mws are 43kD and 35kD respectively. For 43kD isoform, the observed Mw is 53kD.

RRID
AB_2561795 (BioLegend Cat. No. 654201)

Antigen Details

Structure
409 amino acids, predicted molecular weight of 43 kD
Distribution

Nucleus, cytoplasm (the intracellular distribution is cell cycle dependent)

Function
Modulation of proteasomal degradation; involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex
Interaction
Component of the XPC complex composed of XPC, RAD23B and CETN2; interacts with NGLY1, PSMC1, ATXN3, PSMD4, PSMC5, and AMFR
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Ubiquitin/Protein Degradation
Antigen References

1. Sugasawa K, et al. 1997. Mol. Cell Biol. 17:6924.
2. Thoma BS and Vasquez KM. 2003. Mol. Carcinog. 38:1.
3. Reina CP, et al. 2010. Hum. Mol. Genet. 19:235.
4. Farmer LM, et al. 2010. Plant Cell 22:124.
5. Khan O, et al. 2010. Proc. Natl. Acad. Sci. USA 107:6532.
6. Kamiya Y, et al. 2012. FEBS Lett. 586:1141.

Gene ID
5887 View all products for this Gene ID
UniProt
View information about Rad23B on UniProt.org

Related FAQs

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