Purified anti-mouse Galectin-9 Antibody

Pricing & Availability
108A2 (See other available formats)
Regulatory Status
Other Names
Lgals9, Gal-9, Ecalectin
Rat IgG2a, κ
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Product Citations
Western blot analysis of mouse thymus (lane 1), MEF (lane 2), Jurkat (lane 3), and Raji (lane 4) cells using anti-mouse Galectin-9 (clone 108A2) antibody. Purified anti-β-actin (clone Poly6221) was used as loading control.
  • 108A2_PURE_Galectin9_Antibody_1_WB_050316
    Western blot analysis of mouse thymus (lane 1), MEF (lane 2), Jurkat (lane 3), and Raji (lane 4) cells using anti-mouse Galectin-9 (clone 108A2) antibody. Purified anti-β-actin (clone Poly6221) was used as loading control.
  • 108A2_PURE_Galectin9_Antibody_2_IHCF_092016
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse Galectin-9 (clone 108A2) antibody overnight at 4°C followed by 2.5 µg/mL of anti-rat IgG Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
Compare all formats
Cat # Size Price Save
137901 50 µg ¥19,740
137902 500 µg ¥84,420

Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc); Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts and thymus epithelial cells. It may be retained intracellularly or be transported to the cell surface where it is cleaved, thereby generating a soluble form. Galectin-9 is involved in events such as cell aggregation, cell adhesion, chemotaxis and apoptosis. Importantly for the regulation of the immune response, Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Full length recombinant mouse Galectin-9 (M-type)
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
ICFC, IHC-F, WB - Verified
ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 108A2 recognizes the linker peptide of mouse Galectin-9; it does not cross-react with stable Galectin-9 (which is biologically active, genetically engineered, linker-less Galectin-9)3. Additional reported applications (for the relevant formats) include: sandwich ELISA as capture antibody1 and immunohistochemical staining of paraffin2 embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. (ELISA)
  2. Personal communication. (IHC-Paraffin)
  3. Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
  4. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
Product Citations
  1. Sudhakar JN, et al. 2020. Nat Commun. 3.434722222. PubMed
  2. Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
  3. Smith LK, et al. 2021. Elife. 10:. PubMed
  4. Baloche V, et al. 2021. Scientific Reports. 11(1):5227. PubMed
  5. Rebelo C, et al. 2022. Nat Commun. 13:4135. PubMed
AB_10568691 (BioLegend Cat. No. 137901)
AB_10570500 (BioLegend Cat. No. 137902)

Antigen Details

Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)

Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells

Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Cell Type
Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Klibi J, et al. 2009. Blood 113:1957.
2. Seki M, et al. 2008. Clin. Immunol. 127:78.
3. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
4. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.

Gene ID
16859 View all products for this Gene ID
View information about Galectin-9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 09/20/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse Galectin-9

  • PE anti-mouse Galectin-9

  • Alexa Fluor® 594 anti-mouse Galectin-9

  • Alexa Fluor® 488 anti-mouse Galectin-9

  • PE/Cyanine7 anti-mouse Galectin-9

  • APC anti-mouse Galectin-9

  • Alexa Fluor® 647 anti-mouse Galectin-9


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