Purified anti-human/mouse CD3ε (activation epitope) Antibody

Pricing & Availability
APA1/1 (See other available formats)
Other Names
CD3e molecule, epsilon (CD3-TCR complex), CD3e antigen, CD3 epsilon
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes were fixed and permeabilized with 70% ethanol, followed by intracellular staining with purified CD3e (clone APA1/1, filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • APA1_5_Purified_CD3e_Antibody_ICFC_070814
    Human peripheral blood lymphocytes were fixed and permeabilized with 70% ethanol, followed by intracellular staining with purified CD3e (clone APA1/1, filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • APA1_5_Purified_CD3e_Antibody_WB_070814
    Western blot analysis of extracts from Jurkat (lane 1) and PBMC untreated (lane 2) and PBMC activated with CD3/CD28(lane 3) cells using anti-CD3ε (activation epitope) antibody (clone APA1/1). β-actin (poly6221) was used as loading control.
Cat # Size Price Save
362701 50 µg ¥11,000
362702 500 µg ¥44,000

The CD3ε chain of the CD3-TCR complex is a type I membrane protein that undergoes conformational change following ligation of the TCR-CD3 complex.  This conformational change leads to exposure of the proline rich sequence in CD3ε and results in recruitment of Nck. It is expressed by all mature T cells and forms a heteromeric complex with CD3 α/β or γ/δ and ζ chains. It is important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.

Product Details
Technical data sheet

Product Details

Human, Mouse
Antibody Type
Host Species
Purified denatured CD3 proteins from human thymus
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ICFC - Quality tested
WB - Verified
in vivo cell tracking - Reported in the literature, not verified in house
ICC, IHC, IP - Reported by the developer, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For Western blotting, the suggested use of this reagent is 1.0 - 4.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone APA1/1 recognizes an activation-dependent intracellular epitope of the CD3e chain. Additional reported applications (for the relevant formats of this clone) include: immunofluorescent staining of paraformaldehyde fixed sections1, in vivo tracking of T cells 2, and Western blotting3. The LEAF purified format is recommended for in vivo studies. 

Application References

(PubMed link indicates BioLegend citation)
  1. Salmerón A, et al. 1991. J. Immunol. 147:3047. (ICFC, IF)
  2. Risueño RM, et al. 2005. Blood. 106:601. (IF, in vivo tracking, Mouse Reactivity)
  3. Risueño RM, et al. 2008. PLos One. 3:e1747. (WB)
  4. Gil D, et al. 2005. J. Exp. Med. 201:517.
  5. Risueño RM, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:9625.
AB_2563713 (BioLegend Cat. No. 362701)
AB_2563714 (BioLegend Cat. No. 362702)

Antigen Details

Type I membrane protein, part of the TCR-CD3 complex. 207 amino acids, predicted molecular weight of 23 kD.

Mature T cells.

Important role in T cell development and in linking antigen recognition with intracellular signaling pathways.
Interacts with Nck.
MHC antigen.
Cell Type
T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules, TCRs
Antigen References

1. Savage PD, et al. 1988. Cytogenet. Cell. Genet. 49:289.
2. DeJarnette JB, et al. 1998. Proc. Natl. Acad. Sci USA. 95:14909.
3. Wang B, et al. 1999. J. Immunol. 162:88.
4. Szymczak AL, et al. 2005. J. Immunol. 175:270.
5. Borroto A, et al. 2014. J. Immunol. 192:2042.

Gene ID
916 View all products for this Gene ID
View information about CD3epsilon on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 01/15/2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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