- A21001C (See other available formats)
- Regulatory Status
- Other Names
- Protein kinase B alpha, PRK-BA, PKB, Serine/Threonine specific kinase RAC alpha, Protein kinase Akt, RAC
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
AKT (also known as protein kinase B alpha) is a 60 kD serine/threonine-specific kinase containing a pleckstrin domain. AKT plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon cell activation. It is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI 3-kinase. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2, responsible for phosphorylation of AKT at Ser473, has been identified as a mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Activated AKT phosphorylates a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3beta, Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2). AKT promotes cell survival by inhibiting apoptosis through phosphorylation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. AKT also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, AKT phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Host Species
- Synthetic peptide of human AKT phosphorylated at Ser473
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC, ICFC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested by Western Blot using lysates prepared from HeLa cells untreated or treated with IGF-1, and NIH/3T3 cell untreated or treated with PDGF.
This clone is suitable for ICC in cells treated with PFA and permeabilized with either methanol or Triton X-100.
AB_2924618 (BioLegend Cat. No. 606551)
AB_2924618 (BioLegend Cat. No. 606552)
- AKT is a 480 amino acid protein with a predicted molecular weight of 56 kD
Ubiquitously expressed, translocates to the membrane upon activation
- Catalytically inactive multimeric complex. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.
- Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP, CDKN1B.
- Antigen References
- Jacinto E, et al. 2006. Cell. 127:125-37.
- Inoki K, et al. 2002. Nat Cell Biol. 4:648-57.
- Franke TF, et al. 1995. Cell. 81:727-36.
- Brunet A, et al. 1999. Cell. 96:857-68.
- Yang WL, et al. 2010. Cell Cycle. 9:487-97.
- Gene ID
- 207 View all products for this Gene ID
- View information about AKT Phospho Ser473 on UniProt.org
|Purified anti-AKT Phospho (Ser473)||A21001C||WB, ICC, ICFC|
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