- OX-42 (See other available formats)
- Other Names
- Rat C3bi receptor, Rat CR3 complement receptor, ITGAM, ITGAX
- Mouse IgG2a, κ
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- Product Citations
The OX-42 antibody reacts with the CR3 complement (C3bi) receptor expressed on monocytes, granulocytes, macrophages, dendritic cells, and NK cells. This antibody appears to recognize a common epitope shared between CD11b and CD11c (integrin αM and αX chains). The OX-42 antibody precipitates three polypeptides with apparent molecular weights of 160, 103, and 95 kD, respectively. This antibody has been shown to block the formation of complement-mediated rosettes and leukocyte migration.Product Details
- Antibody Type
- Host Species
- Rat peritoneal macrophages.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of C3bi binding3,4.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
- Milligan CE, et al. 1991. J. Comp. Neurol. 314:125. (IHC)
- Robinson AP, et al. 1986. Immunology 57:239. (Block)
- Issekutz SE, et al. 1992. Immunology 76:655. (Block)
- Muehlbauer SM, et al. Am. J Pathol. 177:735. (FC) PubMed
- Thomas RA. et al. 2009. J Pharmacol Toxicol Methods. 60:263. PubMed
- Takeda Y, et al. 2011. Immunobiology. 216:1094. PubMed
- Furuhashi K, et al. 2013. J Am Soc Nephrol. PubMed
- Cheng HY, et al. 2013. Cytotherapy. 1465:608. PubMed
- Product Citations
AB_2565948 (BioLegend Cat. No. 201819)
AB_2565949 (BioLegend Cat. No. 201820)
- Integrin family member
Monocytes, granulocytes, macrophages, dendritic cells
- Receptor for C3bi
- Complement C3bi
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes
- Biology Area
- Cell Biology, Complement, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Robinson AP, et al. 1986. Immunology 57:239.
2. Barcaly AN. 1981 Immunology 42:593.
- Gene ID
- 25021 View all products for this Gene ID 499271 View all products for this Gene ID
- View information about CD11b CD11c on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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