PerCP/Cyanine5.5 anti-mouse IL-9 Antibody

Pricing & Availability
Clone
RM9A4 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
RM9A4_PerCPCy5.5_IL-9_Antibody_FC_1_111913.jpg
Enriched C57BL/6 mouse CD4+ T cells were stimulated with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-β for three days, then re-stimulated with PdBU and ionomycin (in the presence of brefeldin A) for 5 hours, fixed, permeabilized, and then stained with CD4 FITC and IL-9 (clone RM9A4) PerCP/Cyanine5.5 (top) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (bottom).
  • RM9A4_PerCPCy5.5_IL-9_Antibody_FC_1_111913.jpg
    Enriched C57BL/6 mouse CD4+ T cells were stimulated with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-β for three days, then re-stimulated with PdBU and ionomycin (in the presence of brefeldin A) for 5 hours, fixed, permeabilized, and then stained with CD4 FITC and IL-9 (clone RM9A4) PerCP/Cyanine5.5 (top) or rat IgG1, κ PerCP/Cyanine5.5 isotype control (bottom).
  • RM9A4_PerCPCy5.5_IL-9_Antibody_FC_2_111913.jpg
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
514111 25 µg ¥30,000
514112 100 µg ¥70,000
Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant full length mouse IL-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

See the protocol for Th9 polarization of mouse splenocytes.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Angkasekwinai P, et. al. 2010. Nat. Immunol. 11:250. (ICFC) PubMed
  2. Chang HC, et al. 2010. Nat. Immunol. 11:527. (ICFC) PubMed
  3. Takami M, et al. 2012. J Immunol. 188:4369. PubMed.
  4. Reynolds LA, et al. 2012. J. Immunol. 189:1113. PubMed.
RRID
AB_2562729 (BioLegend Cat. No. 514111)
AB_2562730 (BioLegend Cat. No. 514112)

Antigen Details

Structure
Cytokine; 30-40 kD (Mammalian)
Bioactivity
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources
IL-2 activated TH2 lymphocytes
Cell Targets
T lymphocytes, erythroid precursors
Receptors
IL-9R
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V, et al. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y, et al. 1992. Leuk. Lymphoma 8:441.

Regulation
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID
16198 View all products for this Gene ID
UniProt
View information about IL-9 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/22/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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