PE anti-mouse CD45.2 Recombinant Antibody

Pricing & Availability
Clone
QA18A15 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A15_PE_CD45dot2_RECOM_Antibody_051921.png
C57BL/6 mouse splenocytes were stained with anti-mouse CD45.2 recombinant (clone QA18A15) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram).
  • QA18A15_PE_CD45dot2_RECOM_Antibody_051921.png
    C57BL/6 mouse splenocytes were stained with anti-mouse CD45.2 recombinant (clone QA18A15) PE (filled histogram) or rat IgG1, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Save
111103 25 µg ¥13,420
111104 100 µg ¥47,080
Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2892273 (BioLegend Cat. No. 111103)
AB_2892273 (BioLegend Cat. No. 111104)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Antigen References

1. Basu S, et al. 2013. J of Immunol. Methods 396(1-2): 163-167
2. Xu H, et al. 2004. Stem Cells 22: 1039-1048

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05/17/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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