- A2F10 (See other available formats)
- Other Names
- Flk-2, Flt3, Ly-72
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.Product Details
- Antibody Type
- Host Species
- Mouse Flt3 transfected cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
(PubMed link indicates BioLegend citation)
- Sergejeva S, et al. 2004. Blood 103:1270.
- Auffray C, et al. 2009. J. Exp. Med. 206:595.
- Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
- Product Citations
AB_1877218 (BioLegend Cat. No. 135305)
AB_1877217 (BioLegend Cat. No. 135306)
- A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.
- Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
- Flk-2/FLT3 ligand
- Cell Type
- B cells, Hematopoietic stem and progenitors
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478
- Gene ID
- 14255 View all products for this Gene ID
- View information about CD135 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.