PE anti-human CD8a Antibody

Pricing & Availability
Clone
RPA-T8 (See other available formats)
Regulatory Status
RUO
Workshop
IV T171
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-t8
Human peripheral blood lymphocytes stained with RPA-T8 PE
  • RPA-t8
    Human peripheral blood lymphocytes stained with RPA-T8 PE
  • RPA-T8_PE_CD8a_Antibody_042922
    Human peripheral blood was stained with CD8a (clone RPA-T8) PE (solid line) or mouse IgG1, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
Cat # Size Price Save
301007 25 tests ¥7,260
301008 100 tests ¥13,490
301064 100 µg ¥32,120
301051 500 tests ¥61,030
Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  4. Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
  5. Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
  6. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Wang T, et al. 2022. Front Immunol. 13:954121. PubMed
  3. Penkava F, et al. 2020. Nat Commun. 3.76875. PubMed
  4. Zong D, et al. 2021. BMC Biol. 19:79. PubMed
  5. Ding L, et al. 2021. Cancers (Basel). 13:. PubMed
  6. Wu SY, et al. 2021. J Immunother Cancer. 9: . PubMed
  7. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  8. Lo Presti V, et al. 2021. Mol Ther Methods Clin Dev. 21:357. PubMed
  9. Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
  10. Theobald SJ, et al. 2022. EMBO Mol Med. 14:e15888. PubMed
  11. VanSaun M, et al. 2013. PLoS One. 8:73054. PubMed
  12. Mozhgani SH, et al. 2019. Retrovirology. 16:46. PubMed
  13. Mohammadpour H, et al. 2021. Cell Rep. 37:109883. PubMed
  14. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  15. Calzoni E, et al. 2019. J Allergy Clin Immunol. 143:2317. PubMed
  16. Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed
  17. Liu X, et al. 2020. Ther Adv Med Oncol. 12:1758835920910347. PubMed
  18. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  19. Hebbandi Nanjundappa R, et al. 2017. Cell. 171:655. PubMed
RRID
AB_314125 (BioLegend Cat. No. 301007)
AB_314126 (BioLegend Cat. No. 301008)
AB_2564167 (BioLegend Cat. No. 301064)
AB_2562056 (BioLegend Cat. No. 301051)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 08/31/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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