PE anti-human CD109 Antibody

Pricing & Availability
Clone
W7C5 (See other available formats)
Regulatory Status
RUO
Other Names
Gov platelet alloantigen
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W7C5_PE_082808.jpg
Human peripheral blood monocytes stained with W7C5 PE
  • W7C5_PE_082808.jpg
    Human peripheral blood monocytes stained with W7C5 PE
See PE spectral data
Cat # Size Price Save
323305 25 tests ¥20,000
Description

The W7C5 monoclonal antibody recognizes human CD109 also known as the Gov platelet alloantigen. CD109 is a member of the alpha2-macroglobulin/complement gene family that is a GPI-linked cell surface antigen with a predicted molecular weight approximately 162 kD. CD109 contains a thioester motif characteristic of alpha-2-macroglobulin that undergoes autolytic cleavage under denaturing conditions. CD109 expression has been reported on CD34+ and CD34- bone marrow stem cells, CD34+ cells in the fetal liver, CD34+ acute myeloid leukemia cells, T cell lines, activated T lymphoblasts, activated platelets, and endothelial cells. It is also widely expressed in the brain, uterus, heart, lung, and trachea of adults. The function of CD109 is largely unknown although it has been implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and post-transfusion purpura.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WERI-RB-1 retinoblastoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Giesert C, et al. In:Hematopoietic Stem Cells 2002:Genetics and function.
  2. Orlic D, et al. 2003. Ann. New York Acad. Sci. 996:227.
  3. Vogel W, et al. 2002. Haematologica 88:126.
Product Citations
  1. Bojic S, et al. 2018. Stem Cells. 36:1723. PubMed
  2. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
RRID
AB_2073932 (BioLegend Cat. No. 323305)

Antigen Details

Structure
Member of the alpha2-macroglobulin/complement gene family. GPI-linked cell surface antigen, predicted molecular weight approximately 162 kD.
Distribution

Expressed on CD34+ and CD34- bone marrow stem cells, CD34+ cells in the fetal liver, CD34+ acute myeloid leukemia cells, T cell lines, activated T lymphoblasts, activated platelets, and endothelial cells.

Function
Unknown; has been implicated in refractoriness to platelet transfusion, neonatal alloimmune thrombocytopenia, and post-transfusion purpura
Cell Type
Hematopoietic stem and progenitors, Leukemia, Platelets, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Lin M, et al. 2002. Blood 99:1683.
  2. Schuh AC, et al. 2002. Blood 99:1692.
  3. Solomon KR, et al. 2004. Gene 327:171.
Gene ID
135228 View all products for this Gene ID
UniProt
View information about CD109 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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