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A single-cell suspension mixture of 30% human peripheral blood mononuclear cells (PBMCs) with 70% human colorectal adenocarcinoma cell line HT-29 was prepared for positive selection of CD45+ cells using the MojoSort™ Human CD45 Selection Kit. Cells were stained with APC anti-human CD326 (clone CO17-1A) (Cat. No. 369809) and a cocktail of PE conjugated antibodies against CD3/CD14/CD15/CD19/CD56 (clones UCHT1/HCD14/W6D3/HIB19/HCD56) (Cat. Nos. 300407/325605/323005/302207/318305). Dead cells were excluded by Helix NP™ Blue (Cat. No. 425305).
| Cat # | Size | Price | Save |
|---|---|---|---|
| 480167 | 10 tests | ¥43,560 | |
| 480168 | 100 tests | ¥130,900 |
Human CD45+ expressing cells are either selected or depleted by incubating the sample with anti-human CD45 Biotin Antibody followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD45+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage
Kit Contents
- Kit Contents
-
For Cat. No. 480167:
- 1 vial containing 100 µL of anti-human CD45 Biotin-Antibody
- 1 vial containing 100 µL of Streptavidin Nanobeads
For Cat. No. 480168:
- 1 vial containing 1 mL of anti-human CD45 Biotin-Antibody
- 1 vial containing 1 mL of Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Human
- Formulation
-
Cocktail: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Particle: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
-
The antibodies were purified by affinity chromatography, and conjugated
with biotin under optimal conditions. The solution is free of unconjugated
biotin.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads. - Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
10 µL of anti-human CD45 Biotin-Antibody for 1 x 107 cells in 100 µL of buffer.
10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer. - Application Notes
-
This kit is designed for the positive selection or depletion of CD45+ leukocytes from human tissues or tumors.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 4X
Nanobead dilution to use in column: 4X
Antigen Details
- Gene ID
- NA
- UniProt
- View information about CD45 on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
-
The average diameter is approximately 130 nm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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