LEGEND MAX™ Human CD26 (DPP4) ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Depeptidyl Peptidase 4, ADCP2, Adenosine Deaminase Complexing Protein 2
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Product Citations
publications
LEGENDMAX_Human_CD26_DPP4_012323
  • LEGENDMAX_Human_CD26_DPP4_012323
Cat # Size Price Save
449307 1 Pre-coated Plate ¥108,900
Description

CD26, also known as Dipeptidyl peptidase-4 (DPP4), is a serine exopeptidase expressed in many different cell types. It can exist as both a membrane protein and an active soluble form. CD26 is involved in many biological processes including but not limited to cell signaling, immune regulation, and apoptosis. For instance, it can act as a co-stimulatory receptor for T cell activation, while being able to cleave multiple chemokines and peptide hormones such as glucagon, shortening their activity. Due to its abundance and multifunctional characteristic, CD26 can be a biomarker for multiple diseases such as immune disfunction, obesity, diabetes, and cancer.

The LEGEND MAX™ Human CD26 (DPP4) ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with anti-human CD26 (DPP4) antibody. The detection antibody is a biotinylated anti-human CD26 (DPP4) antibody. This kit is specifically designed for the accurate quantitation of human CD26 (DPP4) in serum, plasma, saliva, urine, and cell culture supernatant. It is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human CD26 (DPP4) Pre-coated 96-well Strip Microplate
  • Human CD26 (DPP4) Detection Antibody
  • Human CD26 (DPP4) Lyophilized Standard
  • Avidin HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution D
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Sensitivity
21.3 + 4.5 pg/mL
Standard Range
125 - 8000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Type
Antigen-presenting cells, Epithelial cells, T cells
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Gene ID
P27487 View all products for this Gene ID
UniProt
View information about CD26 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 01/23/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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