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- Regulatory Status
- RUO
- Other Names
- IFNG, IFG, IFI, interferon gamma, Immune interferon
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- Product Citations
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Representative sandwich immunoassay standard curve generated using serial dilutions of LEGEND MAX™ Human IFN-γ Standard ranging from 0.94 pg/mL to 60 pg/mL.
Human IFN-γ (Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-γ, IFN-gamma, etc.) is a potent multifunctional cytokine that is secreted primarily by activated NK cells and T cells. Originally characterized based on its anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Natural IFN-γ is a dimeric protein with subunits of 143 amino acids. IFN-γ is synthesized as a precursor protein of 166 amino acids, including a secretory signal sequence of 23 amino acids. The protein is glycosylated.
The BioLegend LEGEND MAX™ High Sensitivity Human IFN-γ ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human IFN-γ antibody. The detection antibody is a biotinylated mouse monoclonal anti-human IFN-γ antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. Streptavidin-Polymer HRP enhances the sensitivity of the kit, allowing for detection of low concentrations of IFN-γ. This kit is specifically designed for the accurate quantitation of human IFN-γ from serum, heparin plasma, and cell culture supernatant. This kit is analytically validated with ready-to-use reagents.
Kit Contents
- Kit Contents
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- Human IFN-γ Pre-coated 96-well Strip Microplate
- Human IFN-γ Detection Antibody
- Human IFN-γ Lyophilized Standard
- Streptavidin-Polymer HRP
- Assay Buffer A
- 20X Wash Buffer
- Substrate Solution F
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Sensitivity
- 0.267 ± 0.054 pg/mL
- Standard Range
- 0.94 – 60 pg/mL
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Microplate shaker
- Adjustable pipettes to measure volumes ranging from 1 µL to 1000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Polypropylene vials
Antigen Details
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 3458 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org
Related FAQs
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
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We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
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The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
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In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
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