Loading...

HRP Avidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Avidin-Peroxidase, Avidin-Horseradish Peroxidase, Av-HRP
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Save
405103 1 mL ¥28,380
Description

Avidin binds to biotin with high affinity. Avidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For Western blotting or ELISPOT applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.

Product Details
Technical data sheet

Product Details

Formulation
Buffered solution containing bovine protein and preservatives (methylisothiazolone, bromonitrodioxane, and other active isothiazolones).
Preparation
Avidin is conjugated with horseradish peroxidase under optimal conditions.
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
Application

ELISA - Quality tested
ELISPOT, IHC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:5000 to determine optimal conditions. For ELISPOT, dilute at 1:500 - 1:2500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Avidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Av-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.

Application References

(PubMed link indicates BioLegend citation)
  1. Qiu LQ, et al. 2012. J Immunol. 188:5150. PubMed
  2. McLaughlin HP, et al. 2013. J Med Microbiol. 62:185. PubMed
  3. Chan WL, et al. 2013. Nucleic Acids Res. 41:3734. PubMed
  4. Macauley MS, et al. 2014. J Biol Chem. 289:351489. PubMed
  5. Roncolato EC, et al. 2015. Infect Immun. 83:713. PubMed
Product Citations
  1. Clausen TM, et al. 2020. Cell. 183:1043. PubMed
  2. Montégut L, et al. 2022. STAR Protoc. 3:101095. PubMed
  3. Yuan S, et al. 2021. Nature. 593:418. PubMed
  4. Clausen TM, et al. 2020. bioRxiv. . PubMed
  5. Roncolato E, et al. 2015. Infect Immun . 83:713. PubMed
  6. Qiu L, et al. 2012. J Immunol. 188:5150. PubMed
  7. Wu J, et al. 2020. Immunity. 53:115. PubMed
  8. McLaughlin H, et al. 2013. J Med Microbiol. 62:185. PubMed
  9. Chan W, et al. 2013. Nucleic Acids Res. 41:3734. PubMed
  10. Tada R, et al. 2015. PLoS One. 10: e0139785. PubMed
  11. Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed
  12. Macauley M, et al. 2014. J Biol Chem. 289:35149. PubMed
  13. Kojima H, et al. 2021. Scand J Immunol. 93:e13020. PubMed
  14. Narayanasamy A, et al. 2011. J Proteomics. 74:2948. PubMed
  15. Heinzel C, et al. 2021. Front Immunol. 12:753435. PubMed
  16. Salehi S, et al. 2017. PLoS One. 10.1371/journal.pone.0163614. PubMed
  17. Yabe R, et al. 2021. Nat Commun. 0.565277778. PubMed
  18. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/31/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account