Go-ChIP-Grade™ Purified anti-NFATc1 Antibody

Pricing & Availability
Clone
7A6 (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, NFAT2, NFATc
Isotype
Mouse IgG1
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Product Citations
publications
7A6_GoChIP_NFATc1_Antibody_1_120717
Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic kit by loading 3 µg of cross-linked chromatin samples from Raw264.7 cells treated with RANKL with either A) 1:500 dilution of Go-ChIP-Grade™ Purified anti-NFATc1 (Clone 7A6), or B) equal amount of Purified Mouse IgG1 Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting mouse TRAP gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 7A6_GoChIP_NFATc1_Antibody_1_120717
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic kit by loading 3 µg of cross-linked chromatin samples from Raw264.7 cells treated with RANKL with either A) 1:500 dilution of Go-ChIP-Grade™ Purified anti-NFATc1 (Clone 7A6), or B) equal amount of Purified Mouse IgG1 Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting mouse TRAP gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 7A6_GoChIP_NFATc1_Antibody_2_120717
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic kit by loading 3 µg of cross-linked chromatin samples from Raw264.7 cells treated with RANKL with either A) 1:500 dilution of Go-ChIP-Grade™ Purified anti-NFATc1 (Clone 7A6), or B) equal amount of Purified Mouse IgG1 Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting mouse OSCAR gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 7A6_PURE_NFATc1_112817
    Total lysates (15 µg protein) from EL4 (lane 1), HeLa (lane 2), Jurkat (lane 3) and Raw264.7 (lane 4) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-NFATc1 Antibody, clone 7A6 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-NFATc1 Antibody. 7A6 antibody detects different endogenous human NFATc1 spicing variants with an observed molecular weight from 70 - 120 kD. 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody (2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
Cat # Size Price Save
649607 25 µg ¥15,000
649608 100 µg ¥47,000
This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. Nos. 649601, 649602 are available as alternatives.
Description

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. The protein complex consists of NFAT1, NFAT2 (NFATc1 or NFATc), NFAT3, and NFAT4. All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response. NFAT proteins are predominantly expressed in cells of the immune system but are also expressed in skeletal muscle, keratinocytes and adipocytes, regulating cell differentiation programs in these cells. In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus.

Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein of human NFATc1 amino acids 197-304.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Verified
ICC, ICFC - Reported in the literature, not verified in house 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:100-1:2000 by volume. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 4-50, 16-200 immunoprecipitations, respectively, at the recommended dilution.

7A6 antibody detects endogenous human NFATC1 in Western blot. There are 10 isoforms with a predicted MW of 39KD, 74kD, 76kD, 77kD (3), 88kD (2), 100kD, 101kD. With this antibody, observed MW bands range from 70 - 120 kD. The optimal concentration should be determined by titration for each individual assay of interest.

Application References

(PubMed link indicates BioLegend citation)
  1. Timmerman LA, et al. 1997. J. Immunol. 159:2735. (IF)
  2. Brandt C, et al. 2010. Cytometry A. 77:607. (FC)
  3. Fan W, et al. 2012. Arthritis Rheum. 64:3715. PubMed
RRID
AB_2721595 (BioLegend Cat. No. 649607)
AB_2721596 (BioLegend Cat. No. 649608)

Antigen Details

Structure
943 amino acids with predicted molecular weight of 101kD. There are 10 isoforms with a predicted MW of 39kD, 74kD, 76kD, 77kD (3), 88kD (2), 100kD, 101kD.
Distribution

Cytoplasm, Nucleus. NFATc1 is cytoplasmic in the phosphorylated form and nuclear after calcineurin-mediated dephosphorylation controlled activation.

Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Zhao Q, et al. 2010. Int. J. Biochem. Cell Biol. 42:576.
2. Hoey T, et al. 1995. Immunity 2:461.
3. Northrop JP, et al. 1993. J. Biol. Chem. 268:2917.
4. Hogan PG, et al. 2003. Genes Dev. 17:2205.
5. Shaw KT, et al. 1995. P. Natl. Acad. Sci. USA 92:11205.
6. Serfling E, et al. 2007. Sci. STKE 138:pe42.

Gene ID
4772 View all products for this Gene ID
UniProt
View information about NFATc1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 12/08/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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