GMP APC anti-human CD45RA Antibody, CD45RA, HI100

Clone: HI100 (See other available formats)
Workshop: IV N906
Other Names: GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype: Mouse IgG2b, κ
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Product Citations: publications

HI100_GMP_APC_CD45RA_Antibody_030923 — Typical results from human peripheral blood lymphocytes stained either with HI100 APC used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Cat #	Size	Price	Save
260246	100 tests	¥69,400

Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details

Technical data sheet

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration

6 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions², immunohistochemical staining of frozen tissue sections³ and formalin-fixed paraffin-embedded tissue sections⁴, and immunocytochemistry^15,16.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
Palendira U, et al. 2008. Blood (FC) PubMed
Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
Canque B, et al. 2000. Blood 96:3748. (ICC)
Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

RRID

AB_2936658 (BioLegend Cat. No. 260246)

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

Batch-to-batch consistency
Material traceability
Documented procedures
Documented employee training
Equipment maintenance and monitoring records
Lot-specific certificates of analysis
Quality audits per ISO 13485:2016
QA review of released products

Antigen Details

Structure: Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution: B cells, naïve T cells, monocytes
Function: Enhances TCR and BCR signaling
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID: 5788 View all products for this Gene ID
UniProt: View information about CD45RA on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Stem Cells and Development

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45RA Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD45RA	HI100	FC
Biotin anti-human CD45RA	HI100	FC
FITC anti-human CD45RA	HI100	FC
PE anti-human CD45RA	HI100	FC
PE/Cyanine5 anti-human CD45RA	HI100	FC
Purified anti-human CD45RA	HI100	FC,CyTOF®,ICC,IHC-P,IHC-F
Alexa Fluor® 488 anti-human CD45RA	HI100	FC
Alexa Fluor® 647 anti-human CD45RA	HI100	FC,IHC-P
Pacific Blue™ anti-human CD45RA	HI100	FC
Alexa Fluor® 700 anti-human CD45RA	HI100	FC
PerCP/Cyanine5.5 anti-human CD45RA	HI100	FC
PE/Cyanine7 anti-human CD45RA	HI100	FC
APC/Cyanine7 anti-human CD45RA	HI100	FC
Brilliant Violet 421™ anti-human CD45RA	HI100	FC,IHC-P
Brilliant Violet 570™ anti-human CD45RA	HI100	FC
Brilliant Violet 605™ anti-human CD45RA	HI100	FC
Brilliant Violet 650™ anti-human CD45RA	HI100	FC
Brilliant Violet 711™ anti-human CD45RA	HI100	FC
Brilliant Violet 785™ anti-human CD45RA	HI100	FC
Brilliant Violet 510™ anti-human CD45RA	HI100	FC
Purified anti-human CD45RA (Maxpar® Ready)	HI100	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD45RA	HI100	FC
APC/Fire™ 750 anti-human CD45RA	HI100	FC
PerCP anti-human CD45RA	HI100	FC
FITC anti-human CD45RA	HI100	FC
TotalSeq™-A0063 anti-human CD45RA	HI100	PG
Alexa Fluor® 594 anti-human CD45RA	HI100	IHC-P
TotalSeq™-B0063 anti-human CD45RA	HI100	PG
TotalSeq™-C0063 anti-human CD45RA	HI100	PG
Brilliant Violet 750™ anti-human CD45RA	HI100	FC
Spark NIR™ 685 anti-human CD45RA	HI100	FC
APC anti-human CD45RA	HI100	FC
PE/Fire™ 640 anti-human CD45RA	HI100	FC
PE/Fire™ 700 anti-human CD45RA Antibody	HI100	FC
Spark YG™ 581 anti-human CD45RA	HI100	FC
TotalSeq™-D0063 anti-human CD45RA	HI100	PG
Spark Violet™ 423 anti-human CD45RA	HI100	FC
GMP FITC anti-human CD45RA	HI100	FC
PE/Cyanine7 anti-human CD45RA	HI100	FC
PE/Dazzle™ 594 anti-human CD45RA	HI100	FC
APC/Fire™ 750 anti-human CD45RA	HI100	FC
Spark UV™ 387 anti-human CD45RA	HI100	FC
GMP APC anti-human CD45RA	HI100	FC

Concentration & Expiration Lookup

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03/17/2023

For research use only. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.