- Clone
- BM16 (See other available formats)
- Other Names
- Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with CD4 PE, CCR4 PerCP/Cy5.5, and CD294 (clone BM16) FITC (top) or rat IgG2a, κ FITC isotype control (bottom). Data shown was gated on the CD4+ population. -
| Cat # | Size | Price | Save |
|---|---|---|---|
| 350107 | 25 tests | ¥16,000 | |
| 350108 | 100 tests | ¥37,000 |
CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Rat cell line TART/B19-12.10 transfected with human CRTH2
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
This product is sold under license agreement with BML, Inc for research use only.
-
Application References
(PubMed link indicates BioLegend citation) -
- Nagata K, et al. 1999. J. Immunol. 162:1278. (FC)
- De Fanis U, et al. 2007. Blood 109:4343. (FC)
- Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC)
- Hamada K, et al. 2004. Allergology International 53:179. (FC)
- Product Citations
- RRID
-
AB_11204250 (BioLegend Cat. No. 350107)
AB_11204086 (BioLegend Cat. No. 350108)
Antigen Details
- Structure
- Seven-transmembrane protein coupled with G proteins
- Distribution
-
Effector T helper 2 (Th2) cells, eosinophils, basophils
- Function
- Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
- Interaction
- Heterotrimeric G proteins
- Ligand/Receptor
- Prostaglandin D2
- Cell Type
- Th2, Eosinophils, Basophils
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711.
2. Monneret G, et al. 2001. Blood 98:1942.
3. Xue L, et al. 2009. J. Immunol. 182:7580.
4. Schratl P, et al. 2007. J. Immunol. 179:4792. - Gene ID
- 11251 View all products for this Gene ID
- UniProt
- View information about CD294 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
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