APC anti-mouse LAP (TGF-β1) Antibody

Pricing & Availability
TW7-16B4 (See other available formats)
Other Names
Latency Associated Peptide (LAP), Transforming growth factor-beta 1 (TGF-b1), TGFB1, DPD1
Mouse IgG1, κ
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Product Citations
CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes (48 hours) were surface stained with CD4 FITC and LAP (clone TW7-16B4) APC (top) or mouse IgG1, κ APC isotype control (bottom), then intracellularly stained with FOXP3 PE. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_APC_1_062911
    CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes (48 hours) were surface stained with CD4 FITC and LAP (clone TW7-16B4) APC (top) or mouse IgG1, κ APC isotype control (bottom), then intracellularly stained with FOXP3 PE. Data shown was generated by gating on CD4+ lymphocyte population.
  • TW7-16B4_APC_2_062911
See APC spectral data
Cat # Size Price Save
141405 25 µg ¥18,600
141406 100 µg ¥47,700

Transforming growth factor beta (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Mouse Tgfb1-transduced P3U1 cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone TW7-16B4 has been reported to not cross-react with bovine LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 TW7-16B4 recognizes recombinant LAP, latent TGF-β, and pro-TGF-β.

Additional reported applications (for relevant formats) include: Western blotting1 and immunoprecipitation1

Application References

(PubMed link indicates BioLegend citation)
  1. Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB)
  2. Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
Product Citations
  1. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  2. Mukhopadhyay D, et al. 2015. PLoS Negl Trop Dis. 9: e0004145. PubMed
  3. Gubser C, et al. 2016. Sci Rep. 6: 25758. PubMed
  4. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  5. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  6. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  7. Jayachandran R et al. 2018. Immunity. 50(1):152-165 . PubMed
AB_10896418 (BioLegend Cat. No. 141405)
AB_10898159 (BioLegend Cat. No. 141406)

Antigen Details

Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β
Many cell types, highly expressed on activated Tregs and platelets
TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response
TGF-β receptors
Cell Type
Tregs, Platelets, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Oida T, et al. 2010. PLoS One 5:e15523.
2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.
3. Ochi H, et al. 2006. Nat. Med. 12:627.
4. Oida T, et al. 2003. J. Immunol. 170:2516.
5. Nakamura K. 2001. J. Exp. Med. 194:629.
6. Miyazono K, et al. 1993. Growth Factors 8:11.

Gene ID
21803 View all products for this Gene ID
View information about LAP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/27/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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