APC anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
53-6dot7_APC_041106
C57BL/6 mouse splenocytes were stained with CD8 (clone 53-6.7) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • 53-6dot7_APC_041106
    C57BL/6 mouse splenocytes were stained with CD8 (clone 53-6.7) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Save
100711 25 µg ¥6,930
100712 100 µg ¥22,050
Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD8a antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Stone VM, et al. 2022. iScience. 25:105070. PubMed
  2. Zelenka T, et al. 2022. Nat Commun. 13:6954. PubMed
  3. Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed
  4. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  5. Schuster M, et al. 2017. J Immunol. 199:920. PubMed
  6. Li X et al. 2019. Immunity. 50(3):576-590 . PubMed
  7. O'Malley G, et al. 2018. Cancer Immunol Res. 1.240277778. PubMed
  8. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  9. Xu X, et al. 2020. Chin Med. 15:33. PubMed
  10. Choi JG, et al. 2020. Front Immunol. 11:598556. PubMed
  11. Chang YS, et al. 2020. Int J Mol Sci. 21:00. PubMed
  12. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  13. Bian L, et al. 2022. Front Immunol. 13:938598. PubMed
  14. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  15. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  16. Zhao X, et al. 2022. Front Mol Biosci. 9:786864. PubMed
  17. Jiang Q, et al. 2022. Theranostics. 12:59. PubMed
  18. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  19. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  20. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  21. Renrick AN, et al. 2021. Front Immunol. 607044:12. PubMed
  22. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  23. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  24. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  25. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  26. Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed
  27. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  28. Li ZX, et al. 2022. Arch Virol. :. PubMed
  29. Ghosh S, et al. 2022. APL Bioeng. 6:036105. PubMed
  30. Bauer KM, et al. 2022. JCI Insight. 7:. PubMed
  31. Wang D, et al. 2018. Immunity. 48:659. PubMed
  32. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  33. Song C, et al. 2019. Nat Commun. 10:3745. PubMed
  34. Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed
  35. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  36. Riley JS, et al. 2020. Blood Adv. 4:1102. PubMed
  37. Ma Z, et al. 2016. Clin Cancer Res. 22: 2969 - 2980. PubMed
  38. Iwasaki Y, et al. 2016. PLoS One. 11: 0158282. PubMed
  39. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  40. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  41. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  42. Dong L, et al. 2021. Cancer Cell. . PubMed
  43. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  44. Mayer RL, et al. 2022. Nat Commun. 13:6075. PubMed
  45. Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed
  46. del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
  47. Zhao J, et al. 2022. J Nanobiotechnology. 20:62. PubMed
  48. Blumenthal D, et al. 2020. eLife. 9:e55995.. PubMed
  49. Chakrabarti J, et al. 2018. Oncotarget. 9:37439. PubMed
  50. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  51. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  52. Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
  53. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  54. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  55. Zheng H, et al. 2021. Frontiers in Cell and Developmental Biology. 9:641527. PubMed
  56. Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed
  57. Yin Y, et al. 2021. ACS Nano. 15:14347. PubMed
  58. Xu F, et al. 2022. Cell Death Discov. 8:142. PubMed
  59. Chen S et al. 2018. Cell reports. 25(7):1729-1740 . PubMed
  60. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  61. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  62. Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed
  63. Lee JH, et al. 2018. Transgenic Res. 27:241. PubMed
  64. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  65. Felker A, Croy B 2016. J Leukoc Biol. 100: 645 - 655. PubMed
  66. Wagner V, et al. 2016. Sci Transl Med. 8: 366ra164. PubMed
  67. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  68. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  69. Guo H, et al. 2022. PLoS Pathog. 18:e1010857. PubMed
  70. Frey N, et al. 2022. Nat Commun. 13:1804. PubMed
  71. Zhang J, et al. 2022. Front Immunol. 13:931740. PubMed
  72. Bhattacharya P, et al. 2022. PLoS Negl Trop Dis. 16:e0010224. PubMed
  73. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  74. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  75. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  76. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  77. Li Y, et al. 2019. Oncol Rep. 41:2679. PubMed
  78. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  79. Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed
  80. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  81. Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed
  82. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  83. Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
  84. Shade K, et al. 2015. J Exp Med. 212:457. PubMed
  85. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  86. Si L, et al. 2022. Nat Biotechnol. 40:1370. PubMed
  87. Ebina-Shibuya R, et al. 2021. Elife. 10:. PubMed
  88. Solanki A, et al. 2018. Development. 145. PubMed
  89. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  90. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  91. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  92. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
  93. Yin Q, et al. 2015. PLoS One. 10: 0137808. PubMed
  94. Vieyra-Garcia P, et al. 2016. Clin Cancer Res. 22: 3328 - 3339. PubMed
  95. Matsumura K, et al. 2016. J Immunol. 197: 3233 - 3244. PubMed
  96. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  97. He X, et al. 2021. Small. 17:e2007165. PubMed
  98. Pervaiz N, et al. 2021. Pigment Cell Melanoma Res. 34:918. PubMed
  99. Katsinas N, et al. 2022. J Clin Med. 11:. PubMed
  100. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  101. Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed
  102. Brummer G, et al. 2020. Oncogene. 39:2275. PubMed
  103. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  104. Soldevilla MM, et al. 2019. Mol Ther. 27:1878. PubMed
  105. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  106. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  107. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  108. Liu J, et al. 2019. J Exp Med. 216:656. PubMed
  109. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  110. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  111. Divan A, et al. 2018. PLoS One. 13:e0196893. PubMed
  112. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  113. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  114. Poczobutt J, et al. 2016. J Immunol. 196: 891 - 901. PubMed
  115. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  116. Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed
  117. Lee JS et al. 2018. Cell. 174(6):1559-1570 . PubMed
  118. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  119. Zheng Z, et al. 2020. Cell Death Dis. 0.684722222. PubMed
  120. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  121. Chen Q, et al. 2016. Nat Commun. 7:13193. PubMed
  122. Hoefflin R, et al. 2020. Nat Commun. 3.313194444. PubMed
  123. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  124. He X, et al. 2022. Cancer Immunol Res. 10:314. PubMed
  125. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  126. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  127. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  128. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  129. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  130. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  131. Bai C, et al. 2020. Mol Ther Oncolytics. 17:9. PubMed
  132. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  133. Al–Zaher AA, et al. 2018. Mol Ther Oncolytics. 0.376388889. PubMed
  134. Li W, et al. 2019. Nat Commun. 10:3349. PubMed
  135. Budida R, et al. 2017. Eur J Immunol. 47:1819. PubMed
  136. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  137. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
  138. Tartey S, et al. 2021. Cancer Research. 81(9):2358-2372. PubMed
  139. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  140. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  141. Yi X, et al. 2020. Sci Adv. 6:eaba3546. PubMed
  142. Vidy A, et al. 2016. PLoS One. 11:e0165361. PubMed
  143. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  144. Haas MS, et al. 2020. Mol Cancer Res. 19:717. PubMed
  145. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  146. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  147. Li H, et al. 2021. Nat Commun. 12:7149. PubMed
  148. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  149. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  150. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  151. Zhang Q, et al. 2022. Nat Commun. 13:2406. PubMed
  152. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  153. Li YN, et al. 2022. Nat Commun. 13:4074. PubMed
  154. Ren W, et al. 2018. Mucosal Immunol. 12:531. PubMed
  155. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  156. Matsuo K, et al. 2018. J Immunol. 200:800. PubMed
  157. Takaku S, et al. 2020. Integr Cancer Ther. 19:1534735419900798. PubMed
  158. Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
  159. Medyouf H, et al. 2010. Blood. 115:1175. PubMed
  160. Gibbins J, et al. 2014. Blood. 124:2953. PubMed
  161. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  162. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  163. Latasa C, et al. 2016. PLoS One. 11: 0161216. PubMed
  164. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  165. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  166. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  167. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  168. Yang Z, et al. 2021. Onco Targets Ther. 14:4239. PubMed
  169. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  170. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  171. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  172. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  173. Lin W, et al. 2019. EBioMedicine. 49:133. PubMed
  174. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  175. Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed
  176. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  177. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  178. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  179. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  180. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  181. Schreiber K, et al. 2020. Cancer Immunol Res. 8:192. PubMed
  182. Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed
  183. Lin YH, et al. 2018. Cancer Immunol Res. 0.25. PubMed
  184. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  185. Zhang NN, et al. 2020. Cell. 182(5):1271-1283. PubMed
  186. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  187. Sun S, et al. 2013. J Immunol. 190:6209. PubMed
  188. Wang H, et al. 2020. Nat Mater. 1.655555556. PubMed
  189. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  190. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  191. Takahashi F, et al. 2022. iScience. 25:104278. PubMed
  192. Qi J, et al. 2021. Nat Commun. 12:4755. PubMed
  193. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  194. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
  195. Li X, et al. 2021. Cell Death Dis. 12:314. PubMed
  196. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  197. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  198. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  199. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  200. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  201. Wu L, et al. 2020. Cell Rep. 33:108327. PubMed
  202. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  203. Teo T, et al. 2015. J Virol. 89: 7955-7969. PubMed
  204. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  205. Frazer GL, et al. 2021. J Cell Biol. 220:. PubMed
  206. Do-Thi VA, et al. 2021. Cancers (Basel). 13:. PubMed
  207. Wang J, et al. 2022. Clin Transl Med. 12:e718. PubMed
  208. Kataru RP, et al. 2022. Front Aging. 3:864860. PubMed
  209. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  210. Huber MK, et al. 2021. J Vis Exp. :. PubMed
  211. Chen H, et al. 2022. Front Pharmacol. 13:827520. PubMed
  212. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  213. Fonderflick L, et al. 2022. Cells. 11:. PubMed
  214. Rao L, et al. 2020. Nat Commun. 11:4909. PubMed
  215. Murata T, et al. 2020. Sci Rep. 10:13560. PubMed
  216. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  217. Zheng Z, et al. 2021. Nat Commun. 12:6202. PubMed
  218. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  219. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  220. Li X, et al. 2017. Front Immunol. 8:1186. PubMed
  221. Schuster M, et al. 2019. Front Immunol. 10:1583. PubMed
  222. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  223. Fang W, et al. 2021. J Exp Clin Cancer Res. 40:4. PubMed
  224. Zheng Z, et al. 2021. Biomed Res Int. 2021:5535578. PubMed
  225. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  226. Zhang Z, et al. 2020. Nature. 579:415. PubMed
  227. Harrison DK, et al. 2021. Transplantation. 105:540. PubMed
  228. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  229. Zhang J, et al. 2018. Oncoimmunology. 7:e1461301. PubMed
  230. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  231. Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed
  232. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  233. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  234. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  235. Zheng DW, et al. 2020. Nat Commun. 3.865972222. PubMed
  236. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  237. Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
  238. Liu YW, et al. 2022. JCI Insight. 7:. PubMed
  239. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  240. Chen B, et al. 2021. Cell. 184:6262. PubMed
  241. Smith LK, et al. 2021. Elife. 10:. PubMed
  242. Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
  243. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  244. Chakraborty P, et al. 2020. Cell Reports. 28(7):1879-1893.e7.. PubMed
  245. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  246. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  247. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  248. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  249. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  250. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  251. Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed
  252. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  253. Zhou P, et al. 2022. Mol Ther Oncolytics. 25:236. PubMed
  254. Zheng Y, et al. 2022. Transl Res. :. PubMed
  255. Funk KE, et al. 2021. Aging Cell. 20:e13412. PubMed
  256. Konishi Y, et al. 2018. iScience. 10:98. PubMed
  257. Wang J, et al. 2020. J Hematol Oncol. 0.610416667. PubMed
  258. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  259. Zhang Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  260. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  261. Li C, et al. 2022. Front Cell Dev Biol. 10:913824. PubMed
  262. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  263. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
  264. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  265. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
  266. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
RRID
AB_312750 (BioLegend Cat. No. 100711)
AB_312751 (BioLegend Cat. No. 100712)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD8a Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD8a 53-6.7 FC
Biotin anti-mouse CD8a 53-6.7 FC, IHC
FITC anti-mouse CD8a 53-6.7 FC
PE anti-mouse CD8a 53-6.7 FC
PE/Cyanine5 anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a 53-6.7 FC, CyTOF®, IHC-F, IP
PE/Cyanine7 anti-mouse CD8a 53-6.7 FC
APC/Cyanine7 anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 488 anti-mouse CD8a 53-6.7 FC, 3D IHC
Alexa Fluor® 647 anti-mouse CD8a 53-6.7 FC, IHC-F, 3D IHC, SB
Pacific Blue™ anti-mouse CD8a 53-6.7 FC
Alexa Fluor® 700 anti-mouse CD8a 53-6.7 FC
PerCP/Cyanine5.5 anti-mouse CD8a 53-6.7 FC
PerCP anti-mouse CD8a 53-6.7 FC
Brilliant Violet 421™ anti-mouse CD8a 53-6.7 FC, IHC, SB
Brilliant Violet 570™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 650™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 605™ anti-mouse CD8a 53-6.7 FC
Ultra-LEAF™ Purified anti-mouse CD8a 53-6.7 FC, CyTOF®, IHC, IP, Depletion, Block
Brilliant Violet 711™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 785™ anti-mouse CD8a 53-6.7 FC
Brilliant Violet 510™ anti-mouse CD8a 53-6.7 FC
Purified anti-mouse CD8a (Maxpar® Ready) 53-6.7 FC, CyTOF®
Alexa Fluor® 594 anti-mouse CD8a 53-6.7 IHC-F, FC, 3D IHC
PE/Dazzle™ 594 anti-mouse CD8a 53-6.7 FC
APC/Fire™ 750 anti-mouse CD8a 53-6.7 FC
GoInVivo™ Purified anti-mouse CD8a 53-6.7 FC
TotalSeq™-A0002 anti-mouse CD8a 53-6.7 PG
Spark Blue™ 550 anti-mouse CD8a 53-6.7 FC
Spark NIR™ 685 anti-mouse CD8a 53-6.7 FC
TotalSeq™-C0002 anti-mouse CD8a 53-6.7 PG
TotalSeq™-B0002 anti-mouse CD8a 53-6.7 PG
Spark YG™ 570 anti-mouse CD8a 53-6.7 IHC-F
PE/Fire™ 640 anti-mouse CD8a 53-6.7 FC
PE/Fire™ 700 anti-mouse CD8a 53-6.7 FC
Spark Blue™ 574 anti-mouse CD8a Antibody 53-6.7 FC
Spark Violet™ 423 anti-mouse CD8a Antibody 53-6.7 FC
Spark UV™ 387 anti-mouse CD8a 53-6.7 FC
Go To Top Version: 2    Revision Date: 01/21/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD8a

  • Biotin anti-mouse CD8a

  • FITC anti-mouse CD8a

  • PE anti-mouse CD8a

  • PE/Cyanine5 anti-mouse CD8a

  • Purified anti-mouse CD8a

  • PE/Cyanine7 anti-mouse CD8a

  • APC/Cyanine7 anti-mouse CD8a

  • Alexa Fluor® 488 anti-mouse CD8a

  • Alexa Fluor® 647 anti-mouse CD8a

  • Pacific Blue™ anti-mouse CD8a

  • Alexa Fluor® 700 anti-mouse CD8a

  • PerCP/Cyanine5.5 anti-mouse CD8a

  • PerCP anti-mouse CD8a

  • Brilliant Violet 421™ anti-mouse CD8a

  • Brilliant Violet 570™ anti-mouse CD8a

  • Brilliant Violet 650™ anti-mouse CD8a

  • Brilliant Violet 605™ anti-mouse CD8a

  • Ultra-LEAF™ Purified anti-mouse CD8a

  • Brilliant Violet 711™ anti-mouse CD8a

  • Brilliant Violet 785™ anti-mouse CD8a

  • Brilliant Violet 510™ anti-mouse CD8a

  • Purified anti-mouse CD8a (Maxpar® Ready)

  • Alexa Fluor® 594 anti-mouse CD8a

  • PE/Dazzle™ 594 anti-mouse CD8a

  • APC/Fire™ 750 anti-mouse CD8a

  • GoInVivo™ Purified anti-mouse CD8a

  • TotalSeq™-A0002 anti-mouse CD8a

  • Spark Blue™ 550 anti-mouse CD8a

  • Spark NIR™ 685 anti-mouse CD8a

  • TotalSeq™-C0002 anti-mouse CD8a

  • TotalSeq™-B0002 anti-mouse CD8a

  • Spark YG™ 570 anti-mouse CD8a

  • PE/Fire™ 640 anti-mouse CD8a

  • PE/Fire™ 700 anti-mouse CD8a

  • Spark Blue™ 574 anti-mouse CD8a Antibody

  • Spark Violet™ 423 anti-mouse CD8a Antibody

  • Spark UV™ 387 anti-mouse CD8a

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account