APC anti-human IL-2 Antibody, IL-2, W19046A

Clone: W19046A (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-2, T cell growth factor (TCGF); Eosinophil differentiation factor (EDF); Killer cell helper factor (KHF); Macrophage-activating factor for cytotoxicity I (MAF-C I); Thymocyte differentiation factor (TDF)
Isotype: Rat IgG2b, κ
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Product Citations: publications

W19046A_APC_IL-2_Antibody_09052023 — Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (with Brefeldin A) (Cat. Nos. 423303 and 423304) for 6 hours, surface stained with anti-human CD3 PE, and then treated with the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Cells were then stained with anti-human IL-2 (clone W19046A) APC (left) or rat IgG2b, κ APC isotype control (right).

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Cat #	Size	Price	Save
310605	25 tests	¥26,400
310606	100 tests	¥59,400

Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human IL-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
RRID: AB_3083299 (BioLegend Cat. No. 310605)
AB_3083299 (BioLegend Cat. No. 310606)

Antigen Details

Structure: Cytokine; 15.4 kD (Mammalian)
Distribution: T cells
Function: Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Ligand/Receptor: High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.
Gene ID: 3558 View all products for this Gene ID
UniProt: View information about IL-2 on UniProt.org

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Related Protocols

Intracellular Flow Cytometry Staining Protocol

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Other Formats

View All IL-2 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human IL-2	W19046A	ICFC
Spark Violet™ 423 anti-human IL-2	W19046A	ICFC
APC anti-human IL-2	W19046A	ICFC
FITC anti-human IL-2	W19046A	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/05/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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