APC anti-human CD73 (Ecto-5'-nucleotidase) Antibody

Pricing & Availability
AD2 (See other available formats)
Regulatory Status
V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
Human peripheral blood lymphocytes stained with AD2 APC and CD19 PE (top) or mouse IgG1, k APC isotype control and CD19 PE (bottom).
  • A_AD2_APC_091009
    Human peripheral blood lymphocytes stained with AD2 APC and CD19 PE (top) or mouse IgG1, k APC isotype control and CD19 PE (bottom).
  • B_AD2_APC_091009
Compare all formats See APC spectral data
Cat # Size Price Save
344005 25 tests ¥36,520
344006 100 tests ¥73,040

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Reported Reactivity
African Green, Baboon
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations
  1. Ragni E, et al. 2021. Cells. 10: . PubMed
  2. Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed
  3. Jeon SB, et al. 2022. Int J Stem Cells. Online ahead of print. PubMed
  4. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  5. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  6. Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed
  7. Ragni E, et al. 2022. Biomolecules. 12:. PubMed
  8. Santos J, et al. 2013. J Transl Med. 11:18. PubMed
  9. Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
  10. Li J, et al. 2021. Mol Med Rep. 1:23. PubMed
  11. Wang D, et al. 2020. J Cell Mol Med. 24:4212. PubMed
  12. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  13. Xu H, et al. 2022. Cell Death Dis. 13:478. PubMed
  14. Bruveris FF, et al. 2020. Development. 147:00:00. PubMed
  15. Nazari-Shafti TZ, et al. 2020. Biomolecules. 10:00. PubMed
  16. Bordin A, et al. 2019. Stem Cells Int. 2019:1516746. PubMed
  17. Hopf A, et al. 2022. Bioengineering (Basel). 9:. PubMed
  18. Moreno-Fernandez M, et al. 2011. Blood. 117:5372. PubMed
  19. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  20. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  21. Buschmann J, et al. 2013. Cytotherapy. 15:1098. PubMed
  22. Guney-Esken G, et al. 2021. Methods Mol Biol. 2549:23. PubMed
  23. Hofmann L, et al. 2022. Front Med (Lausanne). 9:904295. PubMed
  24. Xu L, et al. 2022. Int J Mol Sci. 23:. PubMed
  25. Siciliano C, et al. 2016. Stem Cell Res. 17: 342-351. PubMed
  26. Ran Y, et al. 2022. Stem Cell Res Ther. 13:507. PubMed
  27. Deng P, et al. 2020. Bone Res. 8:39. PubMed
  28. Safi R, et al. 2021. Photodermatol Photoimmunol Photomed. 37:202. PubMed
  29. Papait A, et al. 2022. Front Immunol. 13:960909. PubMed
AB_1877158 (BioLegend Cat. No. 344005)
AB_1877157 (BioLegend Cat. No. 344006)

Antigen Details

GPI-linked 5'-nucleotidase, 70 kD

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
View information about CD73 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/09/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human CD73 (Ecto-5'-nucleotidase)

  • Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase)

  • Purified anti-human CD73 (Ecto-5'-nucleotidase)

  • PE anti-human CD73 (Ecto-5'-nucleotidase)

  • APC anti-human CD73 (Ecto-5'-nucleotidase)

  • PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)

  • Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase)

  • PerCP/Cyanine5.5 anti-human CD73 (Ecto-5'-nucleotidase)

  • Biotin anti-human CD73 (Ecto-5'-nucleotidase)

  • PE/Dazzle™ 594 anti-human CD73 (Ecto-5'-nucleotidase)

  • APC/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)

  • Brilliant Violet 605™ anti-human CD73 (Ecto-5'-nucleotidase)

  • Brilliant Violet 711™ anti-human CD73 (Ecto-5'-nucleotidase)

  • Brilliant Violet 785™ anti-human CD73 (Ecto-5'-nucleotidase)

  • TotalSeq™-A0577 anti-human CD73 (Ecto-5'-nucleotidase)

  • TotalSeq™-C0577 anti-human CD73 (Ecto-5'-nucleotidase)

  • TotalSeq™-B0577 anti-human CD73 (Ecto-5'-nucleotidase)

  • APC/Fire™ 750 anti-human CD73 (Ecto-5'-nucleotidase)

  • TotalSeq™-D0577 anti-human CD73 (Ecto-5'-nucleotidase)

  • Alexa Fluor® 700 anti-human CD73 (Ecto-5'-nucleotidase)

  • Alexa Fluor® 647 anti-human CD73 (Ecto-5'-nucleotidase)

  • Brilliant Violet 510™ anti-human CD73 (Ecto-5'-nucleotidase)


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account