- Regulatory Status
- RUO
- Other Names
- Lymphocyte function-associated antigen 1, LFA-1, antigen specific, T cells, CD11a/CD18 Lymphocyte function-associated antigen 1, LFA-1, antigen specific, T cells, CD11a/CD18
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
---|---|---|---|
345905 | 10 tests |
This item is currently unavailable |
The Antigen reactive T cell kit contains negative control (vial N) and positive control (vial P) in convenient packaging for stimulating human blood or PBMCs along with anti-human LFA-1 PE and surface stain cocktail consisting of anti-human CD45 PerCP, CD8 Pacific Blue™, CD4 FITC and CD14, CD41, CD15, CD16, CD19 APC/Cyanine7. This kit contains controls that can be paired with peptides of your choice for detecting antigen specific responses as well assessing vaccine responses. Cells or beads stained with individual fluorochrome-conjugated antibodies should be used for setting up compensation before running the samples. For data analysis, we recommend following gating strategy – Time –> Singlets –> Lymphocytes –> APC/Cyanine7 (Dump) negativeCD45+ –> CD4+CD8– and CD8+CD4–.
The Antigen reactive T cell kit provides a convenient, easy to use, and powerful tool for immunology and vaccine research.
Product Details
- Verified Reactivity
- Human
- Formulation
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Each kit will contain the following components:
Kit Components:
1 vial negative control
1 vial positive control
1 empty vial (for peptide stimulation)
1 vial surface stain cocktail (5 tests)
3 tubes of LFA-1 PE (1 test each) 1 bottle 50 mL RBC Lysis/Fixation solution
1 bottle 0.5 mL EDTA 0.08M
1 bottle 500 mL Cell Staining Buffer - Preparation
- Kit consists of lyophilized components.
- Storage & Handling
- Store the kit at 2°C - 8°C upon receipt. Do not open the sealed pouch until ready to use.
- Application
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FC - Quality tested
- Recommended Usage
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Refer to manual for detailed usage instructions.
-
Application References
(PubMed link indicates BioLegend citation) -
- Dimitrov S, et al. 2018. Pro Natl Acad Sci U S A. 115:E5536-E5545.
- Gouttefageas C, et al. 2019. Cancer Immunol Immunother. 68:1855-1863.
- Schollhorn A, et al. 2021. Front Immunol. 12:626308.
- Schollhorn A, et al. 2023. Front Immunol. 13:1107366.
- RRID
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AB_3097144 (BioLegend Cat. No. 345905)
Antigen Details
- Interaction
- CD4+ T cells, CD8+ T cells, antigen specificity
- Ligand/Receptor
- β2-integrin
- Antigen References
-
- Altvater B, et al. 2012. Cancer Immunol Immunother. 61:385.
- Kollgaard T, et al. 2015. 10:e0131934.
- Grant EJ, et al. 2018. Nat Commun. 9:5427.
- Gary R, et al. 2018. J Transl Med. 16:124.
- Gene ID
- NA
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