Alexa Fluor® 647 anti-CHOP Antibody, CHOP, 9C8/CHOP

Clone: 9C8/CHOP (See other available formats)
Regulatory Status: RUO
Other Names: DDIT3, GADD153
Isotype: Mouse IgG2b, κ
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9C8slashCHOP_A647_CHOP_Antibody_ICFC_022823 — THP-1 cells untreated (negative control, open histogram) or treated with 2 µg/mL Tunicamycin for 8 hours (positive control, filled histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Alexa Fluor® 647 anti-CHOP (clone 9C8/CHOP), or Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for untreated and treated cells) (Cat. No. 400330).

Compare all formats See Alexa Fluor® 647 spectral data

Cat #	Size	Price	Save
948703	25 tests	¥43,560
948704	100 tests	¥103,180

Description

When unfolded proteins accumulate in the ER lumen, multiple unfolded protein response (UPR) pathways become activated, including PERK, ATF6, and IRE1α, all of which transcriptionally reprogram the flux of proteins through the ER. When these quality control mechanisms fail to restore ER homeostasis, sustained stress results in the induction of CHOP, a pro-apoptotic transcription factor that negatively regulates expression of BCL2 and increases expression of BIM and TNFS10RB. One mechanism cancer cells exploit to adapt to harsh conditions of the tumor microenvironment is through upregulation of UPR pathways. Thus, understanding the molecular mechanisms that govern the switch from adaptation to apoptosis is of great therapeutic interest.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Partial recombinant human CHOP protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

This clone failed to detect CHOP in human small intestine.

For ICC testing, we do not recommend methanol only as a fixation-permeabilization step due to poor CHOP staining. We recommend 4% PFA fixation with either Triton X-100 or methanol permeabilization.

RRID

AB_2936753 (BioLegend Cat. No. 948703)
AB_2936753 (BioLegend Cat. No. 948704)

Antigen Details

Structure: CHOP is a 169 amino acid protein with a predicted molecular weight of 19 kD.
Distribution: Expressed in cells and tissue undergoing ER stress/Nucleus
Function: Transcription Factor
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Misfolding and Aggregation, Transcription Factors
Antigen References: 1. Yamaguchi, et al. 2004. J. Biol. Chem. 279: 45495
2. Oyadomari, et al. 2004. Cell Death Differ. 11:381.
3. Puthalakath H, et al. 2007. Cell. 129: 1337.
Gene ID: 1649 View all products for this Gene ID
UniProt: View information about CHOP on UniProt.org

Documentation

Reviews

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Related Protocols

Intracellular Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

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Other Formats

View All CHOP Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-CHOP Antibody	9C8/CHOP	WB,ICFC,ICC
Alexa Fluor® 647 anti-CHOP	9C8/CHOP	ICFC
PE anti-CHOP	9C8/CHOP	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02/28/2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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