Recombinant Mouse IL-1α (ELISA Std.)

Pricing & Availability
Regulatory Status
Other Names
RP23-160G19.8, Il-1a
Ave. Rating
Submit a Review
Product Citations
Cat # Size Price Quantity Check Availability Save
580309 4 pack 76€
Check Availability

Need larger quantities of this item?
Request Bulk Quote
Technical Data Sheet (pdf)

Product Details

Mouse IL-1α, amino acids Ser115-Ser270 (Accession # BC003727) was expressed in E. coli.
Molecular Mass
The 156 amino acid recombinant protein has a predicted molecular mass of 17,990 Da. The DTT-reduced and the non-reduced protein migrate at approximately 18kDa by SDS-PAGE. The N-terminal amino acid is Serine.
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.


Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-1a protein is useful as a standard for a mouse IL-1a sandwich ELISA, using unlabeled BL1a-72 antibody (catalog #512402) for capture and biotinylated BL1a-89 antibody (catalog #512504) for detection.

Antigen Details


IL-1α is produced by monocytes, macrophages, keratinocytes, stimulated B lymphocytes, fibroblasts, endothelial cells, and epithelial cells. In addition, IL-1α is localized in various ovarian cell types such as the oocyte, granulosa, and theca cells, and ovarian cancer cells (1).

The cytokine IL-1α is a key molecule both in the innate and in the adaptive immune response. IL-1α induces autonomous proliferation in vascular smooth muscle cells, T cell activation during Ag presentation, up-regulation of monocyte/macrophage-mediated tumor cytotoxicity, stimulation of osteoclast formation, and expression of Cxcl1 (kC) chemokine mRNA and protein in mouse granulosa cells (1,2).
Type I and II IL-1 receptors (IL-1RI and IL-1RII)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Antigen References

1. Soo DS and Roby KF, et al. Mol Endocri 20:2999-3013 2006.
2. Yatabe T, et al. Ann Rheum Dis published online 28 Jul 2008.
3. Ledford H et al, Nature 450:29-30 2007.
4. Boraschi B Tagliabue A et al, Vitam Horm 74:229-254 2006.

Gene ID
16175 View all products for this Gene ID
View information about IL-1alpha on

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/29/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account