Purified anti-SIRT5 Antibody

Pricing & Availability
Clone
O91G9 (See other available formats)
Other Names
Sirtuin 5, SIR2L5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
O91G9_PURE_SIRT5_Antibody_1_060117
Total lysates (15µg protein) from HeLa (Lane 1), 20nM scrambled siRNA (lane 2) and 20nM SIRT5 siRNA (Lane 3) treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:1000 diluted (0.5µg/mL) purified anti-SIRT5 antibody (upper). Proteins were visualized using 1:3000 diluted HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted) was used as a loading control (lower).
  • O91G9_PURE_SIRT5_Antibody_1_060117
    Total lysates (15µg protein) from HeLa (Lane 1), 20nM scrambled siRNA (lane 2) and 20nM SIRT5 siRNA (Lane 3) treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:1000 diluted (0.5µg/mL) purified anti-SIRT5 antibody (upper). Proteins were visualized using 1:3000 diluted HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (1:2000 diluted) was used as a loading control (lower).
  • O91G9_PURE_SIRT5_Antibody_2_060117
    Total cell lysate (15µg protein) from THP-1, HeLa and Raw264.7 cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:250 (2µg/mL), 1:1000 (0.5µg/mL) and 1:2500 (0.2µg/mL) diluted purified anti-SIRT5 antibody (clone O91G9) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody for clone O91G9 or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder, M* indicates longer exposure.
Cat # Size Price Quantity Avail. Save
698801 25 µg 68€
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698802 100 µg 172€
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Description

The seven mammalian Sirtuin isoforms are assigned to different Sirtuin classes on the basis of sequence homology. The mitochondrial SIRT5 belongs to class III. In the mitochondrial matrix, Sirt5 deacetylates and activates carbamoyl phosphate synthetase 1 (CPS1), which is increased when amino acid metabolism is upregulated during fasting. More importantly, SIRT5 regulates lysine succinylation and malonylation as evolutionarily conserved post-translation modifications. SIRT5 was proved to regulate many metabolic pathways, with fatty acid beta-oxidation and ketone body production as two pathways strongly enriched in lysine succinylation. Recently, Zhang et al demonstrated that SIRT5 was targeted to protein complexes on the inner mitochondrial membrane via affinity for cardiolipin in order to promote respiratory chain function.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length protein expressed in 293T cell transfected with human SIRT5 expression vector.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SiRNA knockdown (KD) was used to confirm specificity of this antibody.

RRID
AB_2687321 (BioLegend Cat. No. 698801)
AB_2687322 (BioLegend Cat. No. 698802)

Antigen Details

Structure
310 amino acids, and predicted Mw is 34kDa
Distribution

Mitochondrion

Function
NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically remove malonyl, succinyl and glutaryl groups on target proteins.
Interaction
Interacts with CPS1, cardiolipin. Forms homodimers upon suramin binding.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Mitochondrial Markers
Antigen References

1. Wegner M. 1999. Nucleic Acids Res. 27: 1409.
2. Gertz M, et al. 2016. Cell. Mol. Life Sci. 73:2871.
3. Osborne B, et al. 2016. Free Radic. Biol. Med. 100:164.
4. Nakagawa T, et al. 2009. Cell 137:560.
5. Peng, et al. 2011. Mol. Cell. Proteomics 10:M111.012658.
6. Guan KL and Xiong Y. 2010. Trends Biochem. Sci. 36: 108.
7. Rardin MJ, et al. 2013. Cell. Metab. 18:920.
8. Zhang Y, et al. 2017. J. Biol. Chem. M117.785022.

Gene ID
23408 View all products for this Gene ID
UniProt
View information about SIRT5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/22/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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