Purified anti-rat CD1d Antibody

Pricing & Availability
Clone
WTH-1 (See other available formats)
Other Names
Antigen-presenting glycoprotein CD1d, Cd1d1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
WTH-1_Pure_022712
Lou rat splenocytes were stained with purified CD1d (clone WTH-1, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • WTH-1_Pure_022712
    Lou rat splenocytes were stained with purified CD1d (clone WTH-1, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
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205802 100 µg 88€
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Description

CD1d is a type I transmembrane protein and member of the MHC family that associates with β2-microglobulin on the cell surface. CD1d is expressed by antigen presenting cells (such as dendritic cells, monocytes, macrophages and B cells), thymocytes, and intestinal epithelial cells. CD1d presents glycolipids to Vα14i TCR+ iNKT cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat, Cross-Reactivity: Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
M12.4.1.C3 cells transduced with rat CD1d
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD1d-mediated antigen presentation to type I and II NKT cells1, immunoprecipitation1, Western Blotting1, and immunohistochemical staining of frozen tissue sections1. Clones WTH-1 and WTH-2 recognize different epitopes of CD1d.

Application References
  1. Monzon-Casanova E, et al. 2010. PLoS ONE 5:e13089.
RRID
AB_11147755 (BioLegend Cat. No. 205802)

Antigen Details

Structure
Member of the MHC family, type I transmembrane protein, associates with β2-microglobulin
Distribution

Dendritic cells, monocytes, macrophages, B cells, T cells, thymocytes, intestinal epithelial cells

Function
Presents glycolipid antigens to iNKT cells
Ligand/Receptor
Vα14i TCR
Cell Type
B cells, Dendritic cells, Epithelial cells, Macrophages, Monocytes, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens, TCRs
Antigen References

1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA. 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA. 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.

Gene ID
25109 View all products for this Gene ID
UniProt
View information about CD1d on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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