Purified anti-p63 (ΔN) Antibody

Pricing & Availability
Clone
Poly6190 (See other available formats)
Other Names
p63, δN p63-α, -β, -γ
Isotype
Rabbit Polyclonal IgG
Ave. Rating
6 reviews
Product Citations
publications
Poly6190_p63-deltaN_092811
Non-transfected 293 cell lysates (Lane 1) and p63 (deltaN) transfected 293 cell lysates (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p63 (deltaN) antibody (clone Poly6190). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
  • Poly6190_p63-deltaN_092811
    Non-transfected 293 cell lysates (Lane 1) and p63 (deltaN) transfected 293 cell lysates (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p63 (deltaN) antibody (clone Poly6190). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
619001 50 µl (5 Western blots) 100€
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619002 200 µl (20 Western blots) 280€
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Description

p63 (ΔN) is a member of the p53 family. This protein has multiple isoforms TA p63-α, -β, γ, ΔN p63-α, -β, and -γ. The molecular weights of the p63 isoforms α=66 kD, β=52 kD, and γ=45 kD. This nuclear protein is essential for limb formation, epidermal morphogenesis, and epithelial stem cell regeneration. ΔN isoforms are antagonistic to p53; TA isoforms can transactivate p53 targets. The p63 ΔN isoforms repress TA isoforms; mutant p53 abrogates p63 transactivation. This protein interacts with p53, p73, and the various TA- and ΔN isoforms. The Poly6190 antibody recognizes human p63 ΔN isoforms and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human (recognizes ΔN-isoforms)
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Modified peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References
  1. Medawar A, et al. 2008. PLoS ONE 3:e3441. PubMed
  2. Di Girolamo N, et al. 2009. Transplantation. 87:1571. PubMed
  3. Bray LJ, et al. 2012. Biomaterials. 33:3529. PubMed
  4. Zhou Y, et al. 2011. Clin. Invest. Med. 34:E184. (IHC)
  5. Stewart RM, et al. 2015. Invest Ophthalmol Vis Sci. 56:2021. PubMed
  6. Curtis KM, et al. 2015. PLoS One. 10:123642. PubMed
  7. Ahronian LG, et al. 2015. PLoS One.10:123816. PubMed
Product Citations
  1. Medawar A, et al. 2008. PLoS One. 2.514583333. PubMed
  2. Bray L, et al. 2012. Biomaterials. 33:3529. PubMed
  3. Stewart R, et al. 2015. Invest Ophthalmol Vis Sci . 56:2021. PubMed
  4. Curtis K, et al. 2015. PLoS One. 10:123642. PubMed
  5. Ahronian L, et al. 2015. PLoS One. 10:123816. PubMed
  6. Rode I, et al. 2015. J Immunol. 195: 5678 - 5687. PubMed
  7. Bohnenpoll T, et al. 2016. J Am Soc Nephrol. 2016080849. PubMed
  8. Bohnenpoll T, et al. 2017. PLoS Genet.. 10.1371/journal.pgen.1006951. PubMed
  9. Li Y, et al. 2017. Mol Cancer Res.. 10.1158/1541-7786.MCR-17-0349. PubMed
  10. Lin-Shiao E, et al. 2018. Genes Dev. 8:9905. PubMed
  11. Wang Z 2018. The Journal of cell biology. 217:1941. PubMed
  12. Geyer FC, et al. 2018. Nat Commun. 7:2013. PubMed
  13. Lu Y, et al. 2018. Stem Cells. 36:1875. PubMed
  14. Traver G, et al. 2017. Free Radic Biol Med. 112:578. PubMed
  15. D'Amico S, et al. 2018. Genes Dev. 32:1175. PubMed
  16. Abbas HA, et al. 2018. Cancer Res. 78:451. PubMed
  17. Mamo TM, et al. 2017. Hum Mol Genet. 26:3553. PubMed
  18. Karsli Uzunbas G, et al. 2019. J Biol Chem. 10.1074/jbc.RA119.007965. PubMed
  19. Masterton S, et al. 2019. Biores Open Access. 8:74. PubMed
  20. Aydoğdu N, et al. 2018. Development. 145. PubMed
  21. Hegde GV, et al. 2019. Elife. 8. PubMed
  22. Assmus AM, et al. 2018. Am J Physiol Renal Physiol. 314:F820. PubMed
Publication Library
RRID
AB_2256361 (BioLegend Cat. No. 619001)
AB_2207170 (BioLegend Cat. No. 619002)

Antigen Details

Structure
p53 family, six isoforms, ΔN p63α, β, γ, approximately 66 kD, 52 kD, 45 kD
Distribution
Nuclear
Function
Essential for limb formation, epidermal morphogenesis, epithelial stem cell regeneration. ΔN isoforms antagonistic to p53; TA isoforms can transactivate p53 targets
Interaction
p53, p73, TA- and ΔN isoforms
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Antigen References

1. Yang A, et al. 1998. Mol Cell. 2:305.
2. Celli J, et al. 1999. Cell. 99:143.
3. Gaiddon C, et al. 2001. Mol Cell Biol. 21:1874.
4. Benard J, et al. 2003. Hum Mutat. 21:182.

Regulation
ΔN isoforms repress TA isoforms, mutant p53 abrogates p63 transactivation
Gene ID
8626 View all products for this Gene ID
UniProt
View information about p63 (DeltaN) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10/02/2015

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