Purified anti-NF-κB p65 Antibody

Pricing & Availability
Clone
Poly6226 (See other available formats)
Other Names
NFκB p65, nuclear factor kappa light chain enhancer in B cells p65
Isotype
Rabbit Polyclonal IgG
Ave. Rating
2 reviews
Product Citations
publications
NFkB_p65_060204
Total lysates (15 µg protein) from HeLa (lane 1), Raw 264.7 (lane 2) and UMR106 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.05 µg/mL Purified anti-NF-κB p65 Antibody, clone poly6226 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rabbit-IgG secondary antibody conjugated to HRP for the anti-NF-κB p65 Antibody. 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody (2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • NFkB_p65_060204
    Total lysates (15 µg protein) from HeLa (lane 1), Raw 264.7 (lane 2) and UMR106 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.05 µg/mL Purified anti-NF-κB p65 Antibody, clone poly6226 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-rabbit-IgG secondary antibody conjugated to HRP for the anti-NF-κB p65 Antibody. 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody (2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.
  • poly-p65_101807
    Hela cells stained with purified rabbit polyclonal antibody against NF-κB p65 (poly6226), followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI
Cat # Size Price Quantity Avail. Save
622601 50 µl (5 Western blots) 80€
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622602 200 µl (20 Western blots) 200€
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Description

NF-κB/p65 (nuclear factor kappa light chain enhancer in B cells p65) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p65 is bound (with p50) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF-κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The Poly6226 antibody reacts with the C-terminal region of human, mouse, and rat NF-κB p65 and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat, Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
peptide-KLH, C-terminal
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.1% sodium azide and 0.2% gelatin.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, IHC, IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 µg per ml. For immunofluorescence microscopy, the suggested use of the reagent is a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References
  1. Chen R, et al. 2014. J Mol Cell Cardiol. 72:263. PubMed
  2. Tan GK, et al. 2014. Acta Biomater. 10:2684. PubMed
  3. Abecassis A, et al. 2014. Cell Mol Immunol. 4:377. PubMed
  4. Sumedha N, et al. 2014. Hum Exp Toxicol. 34:506. PubMed
  5. Hua X, et al. 2015. PloS One. 10:128039. PubMed
Product Citations
  1. Ge Y, et al. 2013. J Virol. 87:11160. PubMed
  2. Ayari C, et al. 2011. J Urol. 185:1915. PubMed
  3. Chen R, et al. 2014. J Mol Cell Cardiol. 72:263. PubMed
  4. Tan G, Tabata Y 2014. Acta Biomater. 10:2684. PubMed
  5. Abecassis A, et al. 2014. Cell Mol Immunol. 4:377. PubMed
  6. Miltonprabu N 2014. Hum Exp Toxicol. 34:506. PubMed
  7. Hua X, et al. 2015. PLoS One. 10:128039. PubMed
  8. Yang Z, et al. 2016. Sci Rep. 6:30263. PubMed
Publication Library
RRID
AB_315955 (BioLegend Cat. No. 622601)
AB_315956 (BioLegend Cat. No. 622602)

Antigen Details

Structure
Member of the Rel/dorsal family. One subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits derived from larger precursor proteins
Distribution

Ubiquitously expressed nuclear protein

Function
Transcriptional activator, originally identified as an activator of kappa light chain in B cells
Interaction
Interacts with NF-κB p50 and IκB
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review).
2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).

Regulation
Bound (with p50) to IkB inhibitor in cytoplasm in inactive form. Phosphorylation of IkB releases and NF-kB goes to the nucleus to activate gene expression. Stimulation with LPS, TNF-α, phorbol ester, or IL-1 activates
Gene ID
4790 View all products for this Gene ID
UniProt
View information about NF-kappaB p65 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 01/16/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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