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Purified anti-LEF1 Antibody

Pricing & Availability
Clone
W17021C (See other available formats)
Regulatory Status
RUO
Other Names
Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha (TCF1-alpha), TCF1α, TCF10, TCF7L3
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W17021C_PIRE_LEF1_Antibody_081723
Jurkat cells (positive control) (filled histogram) and Daudi cells (negative control) (open histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with purified anti-LEF1 (clone W17021C), or purified rat IgG2a isotype control (open histogram, dashed line) followed by PE Goat anti-rat IgG (Cat. No. 405406).
  • W17021C_PIRE_LEF1_Antibody_081723
    Jurkat cells (positive control) (filled histogram) and Daudi cells (negative control) (open histogram) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with purified anti-LEF1 (clone W17021C), or purified rat IgG2a isotype control (open histogram, dashed line) followed by PE Goat anti-rat IgG (Cat. No. 405406).
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621051 25 µg 96€
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621052 100 µg 236€
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Description

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream gene expression. LEF1 is implicated as a transcriptional regulator in the transformation of cancer cells in various diseases such as colonic adenocarcinoma. Due to its role in Wnt/β-catenin signaling pathway, LEF1 has been indicated as a facilitator of EMT and potential biomarker for various cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human LEF1 protein corresponding to amino acid residues 116-283
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

LEF-1 is known to have 7 isoforms.

WB (Western blotting) or IHC-P (Immunohistochemistry FFPE) is not suggested for this product. 

For WB, IP (immunoprecipitation) or ICC (immunocytochemistry) applications, please refer to clones 15H5A18 (Cat. No. 653101/653102) or W17155A (Cat. No. 616001/616002). 

For ICFC (intracellular flowcytometry), True-Phos™ Perm Buffer (Cat. No. 425401) is suggested for permeabilization.

RRID
AB_3083406 (BioLegend Cat. No. 621051)
AB_3083406 (BioLegend Cat. No. 621052)

Antigen Details

Structure
LEF1 is a 399 amino acid protein with a predicted molecular weight of 44 kD. Seven total isoforms have been reported, ranging in size from 23 to 44 kD.
Distribution

Lymphoid tissue/Nuclear localization

Function
Canonical Wnt signaling
Interaction
Interacts with ALX4, AML-1, beta-catenin (CTNNB1), EP300, MITF, PIAS4, SMAD2, and SMAD3
Ligand/Receptor
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers, TCRs
Antigen References
  1. Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184-95.
  2. Waterman ML, et al. 1991. Genes Dev. 5:656-69.
  3. Hovanes K, et al. 2001. Nat Genet. 28:53-7..
  4. Bruhn L, et al. 1997. Genes Dev. 11:640-53.
  5. Nguyen A, et al. 2005. Int J Oncol. 27:949-56.
  6. Petropoulos K, et al. 2008. J Exp Med. 205:515-22.
Gene ID
51176 View all products for this Gene ID
UniProt
View information about LEF1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/07/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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